Protein Info for Shew_3136 in Shewanella loihica PV-4

Annotation: FAD linked oxidase domain-containing protein (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 934 PF01565: FAD_binding_4" amino acids 42 to 179 (138 residues), 94.3 bits, see alignment E=2.8e-30 PF02913: FAD-oxidase_C" amino acids 269 to 516 (248 residues), 169.8 bits, see alignment E=4.6e-53 PF13183: Fer4_8" amino acids 539 to 611 (73 residues), 39.9 bits, see alignment 2.5e-13 PF12800: Fer4_4" amino acids 540 to 554 (15 residues), 15.8 bits, see alignment (E = 7.2e-06) amino acids 596 to 610 (15 residues), 13 bits, see alignment (E = 6e-05) PF00037: Fer4" amino acids 540 to 557 (18 residues), 22 bits, see alignment (E = 5.4e-08) PF13237: Fer4_10" amino acids 540 to 609 (70 residues), 28.8 bits, see alignment 5.1e-10 PF13484: Fer4_16" amino acids 541 to 610 (70 residues), 30.5 bits, see alignment 2.7e-10 PF12838: Fer4_7" amino acids 541 to 611 (71 residues), 34 bits, see alignment 1.7e-11 PF13534: Fer4_17" amino acids 541 to 611 (71 residues), 33.3 bits, see alignment 2.9e-11 PF02754: CCG" amino acids 707 to 799 (93 residues), 14.7 bits, see alignment E=1.4e-05 amino acids 834 to 896 (63 residues), 17.3 bits, see alignment 2.2e-06

Best Hits

KEGG orthology group: None (inferred from 100% identity to slo:Shew_3136)

MetaCyc: 82% identical to D-lactate dehydrogenase Dld-II (Shewanella oneidensis)
D-lactate dehydrogenase (cytochrome c-553). [EC: 1.1.2.5]

Predicted SEED Role

"Predicted D-lactate dehydrogenase, Fe-S protein, FAD/FMN-containing" in subsystem Lactate utilization

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.2.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QHQ4 at UniProt or InterPro

Protein Sequence (934 amino acids)

>Shew_3136 FAD linked oxidase domain-containing protein (RefSeq) (Shewanella loihica PV-4)
MSINYSAVVADLRAKLGDEAVSDDAVRRFAWSTDASYFRIVPEVVVHAETLEDARDTLEV
ARQHNAPVTFRAAGTSLSGQAIGEGILLILGHDGFRKIAVSEDHNQISLGAAVIGGDANL
ALKPFNKKIGPDPATLASAMVGGIVSNNASGMCCGTAQNSYQTIASAKLLFADGTRLDTG
CERSKEAFRESHQQLLSELTDLAKLTASNPVLADRIRKKYSIKNTTGYSINALVDFEDPF
ELINHLIVGAEGTLAFVEEVTYNTVDEAKFKASAMAVFFNMEDAARAIPPIKCDSVAAAE
LLDWASIKAVTGKKGMPDWLSELPEGAAILLIESRANDEATLDAYTQDVIAKLAHIETER
PISFSRDPEVYSKYWAMRSGLFPIIGGERPKGTSVIIEDVAFELEHLANAATDLTALFHK
HGYPEGVIYGHALAGNFHFIITPTFASQEDIDRFHGFMQDVAEMVINKYDGSMKAEHGTG
RAVAPFVEMEWGADAYTLMKRIKQIFDPQGLLNPGVILNDDANVHVKNIKPCPVVDDFVD
RCIECGFCEKTCPTSALNFTPRQRIATLREIARLEASGDKQAAEEMRAAAKYDVVDTCAA
CQLCTIACPVDNSMGQLVRKLRTPYITTTEQKVLDFQAKHFGAVNQVISTGFDVLSIIHK
VTGDSVTNGLMKVGRMVSKEVPYWNPDFAKGGKLPKPSPASPDKETVVYFPACGGRTFGP
TPKDPDNRTLPEVVVTLLERAGYNVVTPDNTRHLCCGQMWESKGDFKNADAKRDELIDAL
SALSDNGKVPVVVDALSCTYRTLTGNPKVEITDLVEFMHDKILPKLTFTKKVNVALHLGC
SARKMKLEPKMQALADACSNGVKLPEGIECCGYAGEKGLYKPEINASALRNIKKLIPVDI
KEGYYANRMCEVGLTQHSGISYRHLAYLLEECSR