Protein Info for Shew_3037 in Shewanella loihica PV-4

Annotation: alpha-L-glutamate ligase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 317 PF18030: Rimk_N" amino acids 1 to 94 (94 residues), 76.2 bits, see alignment E=4.1e-25 TIGR00768: alpha-L-glutamate ligase, RimK family" amino acids 2 to 287 (286 residues), 199.3 bits, see alignment E=4.1e-63 PF08443: RimK" amino acids 98 to 287 (190 residues), 158.5 bits, see alignment E=3e-50 PF02655: ATP-grasp_3" amino acids 99 to 261 (163 residues), 24 bits, see alignment E=7.7e-09 PF02955: GSH-S_ATP" amino acids 114 to 263 (150 residues), 49.8 bits, see alignment E=5.6e-17

Best Hits

Swiss-Prot: 39% identical to RIMK_COXBN: Probable alpha-L-glutamate ligase (rimK) from Coxiella burnetii (strain Dugway 5J108-111)

KEGG orthology group: None (inferred from 100% identity to slo:Shew_3037)

Predicted SEED Role

"Ribosomal protein S6 glutaminyl transferase" in subsystem Ribosome biogenesis bacterial

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QHF5 at UniProt or InterPro

Protein Sequence (317 amino acids)

>Shew_3037 alpha-L-glutamate ligase (RefSeq) (Shewanella loihica PV-4)
MKIAILTTNQVDDDNRLVEVARAKGHDAFLLDLRQISIELLPSNPEIYYQNEVITNQFDI
VIPRLNVSYTDFGINILQQFICAGIYVSESPEALRLGRDKLKCLQYLLSKDLPFPATAIA
YTPEYLETLVGHLKLPLVVKLIESTEGTGVFLAKTKKEVDNLCKTFSLAGVSYQIQEFIA
EAAGQDIRAFVVGDRVVSAMQRESQDDDFRANVSLGAHSAAVQLTPEEEQVVLRATQSIG
VNIAGVDFVRSSRGPLLLEINVSPDFTGKQGLETVTNCNIAAAIIDYTLSEAKGFYRHQR
LMASRVRREEDLLCADG