Protein Info for Shew_3000 in Shewanella loihica PV-4

Annotation: diguanylate cyclase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 585 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details transmembrane" amino acids 376 to 393 (18 residues), see Phobius details TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 403 to 566 (164 residues), 155 bits, see alignment E=7.2e-50 PF00990: GGDEF" amino acids 406 to 563 (158 residues), 141.3 bits, see alignment E=1.2e-45

Best Hits

KEGG orthology group: None (inferred from 100% identity to slo:Shew_3000)

Predicted SEED Role

"GGDEF family protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QHB8 at UniProt or InterPro

Protein Sequence (585 amino acids)

>Shew_3000 diguanylate cyclase (RefSeq) (Shewanella loihica PV-4)
MKVRYLLLLLIFFFSQSMAEEVDIDATLTQADQLKSSNPKAFSELLNTLEKEPLNPQQRY
FLDYLRGYELTYQGKQAQSIDVFNKILNSNANETLKFRANQTLINIYAISQDWSKGLTHL
SKNFQFIEQIKDPETVQSGLAIAAIFYNQIEQYELGLSYAEKLRSSKPSKRNTCLANGLA
IEAQLGLNQLEVDSPSLQAAVASCDEEVIMESFIHSFIATKYLRDGNTAEVYGQLKPSLE
TIEKTRYPRLIAEVYATLASAYLQDHKIGEARLLAEKIVNLAKGLGNTQAVVTAYKVLYQ
IAELSGNYKAALDYHVKYAQADKAYLDDIKTKHLAFQLAEHQAAEQKSRIALLDRQNNLL
LTEQQLAKTQAENNRLFISLLIAIITLLGFWAYKSWTIQKRLKQLAEYDALTHVFNRGHF
TQVAQSALTYCESSEVDLSYILFDLDNFKQINDKYGHAIGDWVLKKVAKACQAQGRKNDI
FARIGGEEFCIALPGCDLQTAVKLAEACRKAIAGIDCGATGHEFEVTASFGITDTQMSGY
KLERLINDADSAMYQSKETGRDRICIFDPEAKRTIPQASHSLLSL