Protein Info for Shew_2986 in Shewanella loihica PV-4

Annotation: 5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF08267: Meth_synt_1
  • amino acids 8 to 312 (305 residues), 380 bits, E=1.1e-117
  • amino acids 551 to 688 (138 residues), 29.7 bits, E=5e-11
• TIGR01371: 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase
  • amino acids 9 to 756 (748 residues), 1051.6 bits, E=0
• PF01717: Meth_synt_2
  • amino acids 430 to 752 (323 residues), 493.4 bits, E=3.4e-152

Best Hits

Swiss-Prot: 74% identical to METE_SHEON: 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K00549, 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase [EC: 2.1.1.14] (inferred from 100% identity to slo:Shew_2986)

Predicted SEED Role

"5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (EC 2.1.1.14)" in subsystem Methionine Biosynthesis (EC 2.1.1.14)

MetaCyc Pathways

• aspartate superpathway (24/25 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (18/18 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (9/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (9/9 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (11/12 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (8/8 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
• L-methionine biosynthesis I (5/5 steps found)
• S-adenosyl-L-methionine salvage I (4/4 steps found)
• L-methionine biosynthesis II (5/6 steps found)
• L-methionine biosynthesis III (3/4 steps found)
• S-adenosyl-L-methionine salvage II (2/3 steps found)
• seleno-amino acid biosynthesis (plants) (3/5 steps found)
• superpathway of seleno-compound metabolism (8/19 steps found)

KEGG Metabolic Maps

• Biosynthesis of plant hormones
• Methionine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.14

Sequence Analysis Tools

See A3QHA4 at UniProt or InterPro

Protein Sequence (759 amino acids)

>Shew_2986 5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase (RefSeq) (Shewanella loihica PV-4)
MQTLDIASLGFPRIGRQRELKFALEKYWRGEIEQTQLQSLASELRRTHWQWQQAAGVKQV
PVGDFAFYDQVLTLSATINAIPARHRSGEQVDLDTLFRVARGRAPTGEASRAAQMKKYFN
TNYHYIVPELTAGQRFEIAYEQLFDEVAEAKSLGHQPKPVLLGPVSYLYLAKCEGEPFDK
LSLLPNLLEAYQAILARFADQGVTWVQLDEPILALELDDDWLAAFEPAYKELSGQGVKLL
LASYYGSVAHHHELIKDLVVDGLHLDLVSDAEQLTLFGASLKPTQVLSLGVINGRNVWAA
DLDILAERLRGLVADLGERVWLAPSCSLLHVPVDLESETELAPELANQLSFAKQKLDELA
ALRTLLLTPQSEAAQAIVARCEARRQVREQAANPEVQARLKALTPEDFNRPSGFGERRLK
QQARFRLPLLPTTTIGSFPQTPAIRGLRSRWRKGELTEQAYVAQLEAVTRDSIARQLKLG
IDVLVHGEAERNDMVEYFGEQLDGVGFTQFAWVQSYGSRCVKPPLIYGDVSRRQPMTLAW
ASFAQGLTDRPVKGMLTGPVTILHWSFAREDIGREQIANQLALAIRDEVADLQAAGIGII
QIDEPAFREGLPLKASDWKHYLDWAVAAFKLSAAGAEDETQIHTHMCYSEFNATIDAIAA
MDADVITIETSRSHMELLNAFEDFEYPNEIGPGVYDIHSPNIPTVEQMVALIEKAATKIP
VGQLWVNPDCGLKTRTWDEVEPALQNMVKATKELRRRLG