Protein Info for Shew_2977 in Shewanella loihica PV-4

Annotation: diguanylate cyclase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 signal peptide" amino acids 1 to 48 (48 residues), see Phobius details transmembrane" amino acids 455 to 476 (22 residues), see Phobius details PF13424: TPR_12" amino acids 176 to 245 (70 residues), 30.7 bits, see alignment E=5.8e-11 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 487 to 646 (160 residues), 159.9 bits, see alignment E=2.3e-51 PF00990: GGDEF" amino acids 489 to 644 (156 residues), 145.5 bits, see alignment E=2.5e-46

Best Hits

KEGG orthology group: None (inferred from 100% identity to slo:Shew_2977)

Predicted SEED Role

"GGDEF domain protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QH95 at UniProt or InterPro

Protein Sequence (650 amino acids)

>Shew_2977 diguanylate cyclase (RefSeq) (Shewanella loihica PV-4)
MLWLNGHIARLAKLKMTRTTKAKSWITSLLLISLSLSLLGLSPLSQAQEDSAEEIDAIFK
KFDQGEYKDQQAIKEALGYLTANITADDMERYLKLQTVLCWNNYDITKQQALKDAIAFAD
LKLLTEGVAASAEATTDLKLCRAFMHQLLGKVDIALEEYNKVVAEAYLIESPRLIADSRS
LRGAIYSFQGNFAQALEDLITAQHLYESLNLQHWAIYNLTELATSYRRFGDPQTAIKYYR
KLEKGFEDTGDKDGANAIKTEIGFALEELGEYEAALKKHKEAYQYWRDTLGEEASAHTAV
NVAGVLIQLHRQGEAKPYLDAAQKSITPDQGASYSFMRLYQAQSALTNGQYQQALDYIDM
AEETFKQIKNARGLEQLHMVHSQIFVAEKDWKQAYEALLSYIQTHKSLDATQQTNRTTEM
RTRFNTEQIERENQQLLELQKIKENELVILKQNKYLQLAVIVLGCIIMIILSIVAYKQSQ
KSKLLSILALTDHLTQLPNRRYTYSKGEGYFKSKDEGHQPLSIILFDADHFKKVNDKYGH
DIGDKVLIALANISNGLMRKQDLVGRVGGEEFLVILPGTTAEQALNVAQRLVTTVESSNF
DDVYMNFKLTISAGIACTDGDKSFEHLLKRADDALYRAKSSGRNCAILDT