Protein Info for Shew_2827 in Shewanella loihica PV-4

Name: infB
Annotation: translation initiation factor IF-2 (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 885 PF04760: IF2_N" amino acids 1 to 47 (47 residues), 42 bits, see alignment 2.8e-14 amino acids 310 to 360 (51 residues), 64.3 bits, see alignment 3.2e-21 PF08364: IF2_assoc" amino acids 59 to 97 (39 residues), 58 bits, see alignment (E = 4e-19) TIGR00487: translation initiation factor IF-2" amino acids 302 to 885 (584 residues), 948.7 bits, see alignment E=1.5e-289 TIGR00231: small GTP-binding protein domain" amino acids 389 to 541 (153 residues), 109.4 bits, see alignment E=1.6e-35 PF00009: GTP_EFTU" amino acids 389 to 545 (157 residues), 126.2 bits, see alignment E=5.4e-40 PF01926: MMR_HSR1" amino acids 390 to 495 (106 residues), 39.7 bits, see alignment E=2.2e-13 PF00071: Ras" amino acids 391 to 545 (155 residues), 21.7 bits, see alignment E=6.1e-08 PF22042: EF-G_D2" amino acids 561 to 639 (79 residues), 104.9 bits, see alignment E=9.1e-34 PF11987: IF-2" amino acids 661 to 775 (115 residues), 150.5 bits, see alignment E=8.1e-48 PF03144: GTP_EFTU_D2" amino acids 806 to 873 (68 residues), 34.6 bits, see alignment 9.3e-12

Best Hits

Swiss-Prot: 72% identical to IF2_ALIFM: Translation initiation factor IF-2 (infB) from Aliivibrio fischeri (strain MJ11)

KEGG orthology group: K02519, translation initiation factor IF-2 (inferred from 71% identity to aha:AHA_3303)

Predicted SEED Role

"Translation initiation factor 2" in subsystem NusA-TFII Cluster or Translation initiation factors eukaryotic and archaeal or Universal GTPases

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QGU5 at UniProt or InterPro

Protein Sequence (885 amino acids)

>Shew_2827 translation initiation factor IF-2 (RefSeq) (Shewanella loihica PV-4)
MADTTVAKLAEEVGKSADRLVEQFSEAGIKKSKTDTVSEDEKQKLLEFLKKQHGGDSAPT
KMTLQRKSISTLSVSGSGGQSKDIKVEVRKKRTFVKRDVAAEAEAEAKAKAEAEAKAAAE
AEAKAKADAEAKAKAEAEAAAKAKAKAEAEAKAKKAAEGKPAAEETAEEKAAKVEEARLL
AAKEAAAKAKADEEAKAAAEEARRLAEENEKRWAEEERLRKESENSADHHVTTSTEARAA
EDSADRDAERRGRRARKPAGGKKEPIGKKRGGKEARGGRNNRNQRNAPESMDHGFNKPAA
AVTRDVTIGETVTVAELAQKMAVKATEIIKQMMKMGSMVTINQVLDQETAQLVAEEMGHK
VVLTRENELEHQVLADRDGNVKVEPRAPVVTIMGHVDHGKTSLLDYIRRAKVASGEAGGI
TQHIGAYHVETDNGMITFLDTPGHAAFTAMRARGAKATDIVVLVVAADDGVMPQTIEAIQ
HAKAGGVPLIVAVNKIDKPEADPDRVKSELSQHGVMSEDWGGNNMFVHVSAKSGEGIDEL
LEGILLEAEVLELKAVREGMAAGVVVESKLDKGRGPVATILVQEGTLKQGDIVLCGLEYG
KVRAMKDENGKSITEAGPSIPVEILGLSGVPSAGDEATVVRDERKAREVALYRQGKFRDV
KLARQQKSKLENMFANMTEGEVQELNIVLKADVQGSLEAISDSLNKLSTDEVKVNIIASG
VGGLTETDATLAAASNAIMVGFNVRADAQARKTIESESVDLRYYSVIYHLIDEVKAAMGG
MLAPEFKQEIIGLAEVRDVFKSPKIGAIAGCMVTEGTIKRSAPIRVLRDNVVIFEGELES
LRRFKDDVNEVRNGMECGIGVKNYNDVKVGDQIEVFETVEIARTL