Protein Info for Shew_2764 in Shewanella loihica PV-4

Annotation: DNA-binding transcriptional activator GcvA (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 303 PF00126: HTH_1" amino acids 8 to 67 (60 residues), 80.2 bits, see alignment E=8.6e-27 PF03466: LysR_substrate" amino acids 93 to 291 (199 residues), 121.8 bits, see alignment E=2.7e-39

Best Hits

Swiss-Prot: 71% identical to GCVA_ECOLI: Glycine cleavage system transcriptional activator (gcvA) from Escherichia coli (strain K12)

KEGG orthology group: K03566, LysR family transcriptional regulator, glycine cleavage system transcriptional activator (inferred from 100% identity to slo:Shew_2764)

Predicted SEED Role

"Glycine cleavage system transcriptional activator GcvA" in subsystem Glycine cleavage system or Orphan regulatory proteins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QGN2 at UniProt or InterPro

Protein Sequence (303 amino acids)

>Shew_2764 DNA-binding transcriptional activator GcvA (RefSeq) (Shewanella loihica PV-4)
MSRRLPPLNAVKAFEAAARHLSFTRAAEELFVTQAAVSHQIKALEEYLGLKLFRRKNRSL
LLTEEGQGYFLDIKDIFVQLADATDRLLARSAIGSLTVSTSPSFAIQWLVPRLANFSENN
PDIDVRIKAVDDETSSLTDDVDVAIYYGMGNWPGLRADKLRNEVLIPVCSPMLLSGPKPL
SQPSDLKHHTLLHDSSRHDWQAWFRQCGINDINVNQGPIFSHSSLVLQAAAHGQGVALGY
SVLARPDIKAGRLVCPFPEVLVSKNAYYLVCQQNHAEIGKIVAFRKWMLDMFEEESRSEL
LPG