Protein Info for Shew_2668 in Shewanella loihica PV-4

Annotation: 2-nitropropane dioxygenase, NPD (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF18328: PfaD_N
  • amino acids 15 to 80 (66 residues), 57.5 bits, E=1.6e-19
• TIGR02814: PfaD family protein
  • amino acids 83 to 521 (439 residues), 710 bits, E=5.7e-218
• PF03060: NMO
  • amino acids 95 to 382 (288 residues), 30.2 bits, E=4.7e-11
• PF21607: FabD_helical_ins
  • amino acids 397 to 476 (80 residues), 121.3 bits, E=2.3e-39

Best Hits

KEGG orthology group: None (inferred from 100% identity to slo:Shew_2668)

MetaCyc: 85% identical to icosapentaenoate synthase D subunit (Shewanella pneumatophori)
2.3.1.-

Predicted SEED Role

"Enoyl-[acyl-carrier-protein] reductase [FMN] (EC 1.3.1.9), inferred for PFA pathway" (EC 1.3.1.9)

MetaCyc Pathways

• superpathway of fatty acids biosynthesis (E. coli) (50/53 steps found)
• palmitate biosynthesis II (type II fatty acid synthase) (29/31 steps found)
• superpathway of fatty acid biosynthesis II (plant) (37/43 steps found)
• superpathway of unsaturated fatty acids biosynthesis (E. coli) (20/20 steps found)
• biotin biosynthesis I (14/15 steps found)
• oleate biosynthesis IV (anaerobic) (13/14 steps found)
• palmitoleate biosynthesis I (from (5Z)-dodec-5-enoate) (9/9 steps found)
• 8-amino-7-oxononanoate biosynthesis I (10/11 steps found)
• (5Z)-dodecenoate biosynthesis I (6/6 steps found)
• superpathway of fatty acid biosynthesis I (E. coli) (13/16 steps found)
• cis-vaccenate biosynthesis (5/5 steps found)
• gondoate biosynthesis (anaerobic) (4/4 steps found)
• (5Z)-dodecenoate biosynthesis II (5/6 steps found)
• stearate biosynthesis II (bacteria and plants) (5/6 steps found)
• 8-amino-7-oxononanoate biosynthesis IV (4/5 steps found)
• fatty acid elongation -- saturated (4/5 steps found)
• icosapentaenoate biosynthesis IV (bacteria) (1/1 steps found)
• anteiso-branched-chain fatty acid biosynthesis (24/34 steps found)
• even iso-branched-chain fatty acid biosynthesis (24/34 steps found)
• odd iso-branched-chain fatty acid biosynthesis (24/34 steps found)
• streptorubin B biosynthesis (20/34 steps found)
• mycolate biosynthesis (20/205 steps found)
• superpathway of mycolate biosynthesis (21/239 steps found)

KEGG Metabolic Maps

• Fatty acid biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 1.3.1.9

Use Curated BLAST to search for 1.3.1.9

Sequence Analysis Tools

See A3QGD6 at UniProt or InterPro

Protein Sequence (545 amino acids)

>Shew_2668 2-nitropropane dioxygenase, NPD (RefSeq) (Shewanella loihica PV-4)
MNPTTTNEQLSPWPWQVSDSKISFDTAAMATALKDFSRACYLVNDSDLGVGIATEASLMT
NNQGSGAQASGHPVSAFTPALGTESLGDGNFRRVHGVKYAYYAGAMANGISSEELVIALG
KAGILCSFGAAGLIPSRVEQAIARIQTALPNGPYMFNLIHSPSEPALERGSVELFLKHKV
RTVEASAFLGLTPQIVYYRAAGLSRDANGHIIIGNKVIAKVSRTEVAEKFMQPAPVKMLQ
KLVDEGLITHDQMEMAQTVPMADDITAEADSGGHTDNRPLVTLLPTILALKEEIQDKYQY
ATPLRVGCGGGIGTPDAALAAFNMGAAYIVTGSINQSCVEAGASEHTRKLLATTEMADVT
MAPAADMFEMGVKLQVVKRGTLFPMRANKLYEIYSRYDSIEAIPVDERDKLEKQVFRASL
DEIWAGTVAHFNERDPKQIERAEGNPKRKMALIFRWYLGLSSRWSNTGEQGREMDYQIWA
GPALGAFNQWAKGSYLDDYSKRHAVDLAKHLMYGAAYLARVNAIKAQGIKLPGELLRYKP
QVALA