Protein Info for Shew_2573 in Shewanella loihica PV-4

Annotation: carbamoyl-phosphate synthase L chain, ATP-binding (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 677 PF00289: Biotin_carb_N" amino acids 1 to 110 (110 residues), 154.5 bits, see alignment E=4.6e-49 PF02786: CPSase_L_D2" amino acids 116 to 327 (212 residues), 258 bits, see alignment E=2.2e-80 PF02222: ATP-grasp" amino acids 134 to 279 (146 residues), 29.1 bits, see alignment E=2.6e-10 PF07478: Dala_Dala_lig_C" amino acids 139 to 296 (158 residues), 28.4 bits, see alignment E=4e-10 PF02785: Biotin_carb_C" amino acids 341 to 449 (109 residues), 128 bits, see alignment E=5.9e-41 PF00364: Biotin_lipoyl" amino acids 605 to 670 (66 residues), 59.1 bits, see alignment E=1.1e-19

Best Hits

KEGG orthology group: K01968, 3-methylcrotonyl-CoA carboxylase alpha subunit [EC: 6.4.1.4] (inferred from 100% identity to slo:Shew_2573)

Predicted SEED Role

"Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4)" in subsystem HMG CoA Synthesis or Leucine Degradation and HMG-CoA Metabolism or Serine-glyoxylate cycle (EC 6.4.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.4.1.4

Use Curated BLAST to search for 6.4.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QG41 at UniProt or InterPro

Protein Sequence (677 amino acids)

>Shew_2573 carbamoyl-phosphate synthase L chain, ATP-binding (RefSeq) (Shewanella loihica PV-4)
MFTKLLIANRGEIACRIIKTAQVMGIKTVALYSDADKDARHVAMADESFYLGGSAPSESY
LKGELILEIAKRSGAQAIHPGYGFLSENADFARQCEQAGIAFVGPSADAIDAMGSKSAAK
IIMEQANVPLVPGYHGDDQSDEVLLKASKEIGFPQLIKAAYGGGGKGMRIVESESEALEA
INSARREAASSFGNDKLLIERYLRQPRHVEVQVFADTQGNCIYLSDRDCSIQRRHQKVVE
EAPAPGLSDSLRRAMGEAAVAAAKAIDYVGAGTVEFLLDTHATEESNSFYFMEMNTRLQV
EHPVTEMVTGQDLVKWQLMVASGSELPLSQEDVRIHGHSFEVRIYAEDPQNEFLPASGKL
NFLREPEQNRYVRIDSGIRENDVISNFYDPMIAKLIVWDESRPRALQRLVHALESYQISG
LKHNIEFLANIAEHKAFSQADFSTDFIERYGDTLIGANLDGDNSHEQNGALAFAALYHLC
AQKEQAKLTATNSQDPYSPWGQVSGFRLNRSGMHQLSLLDDEHEVQHLTLSQIGDCYYLN
LNDQQLTLSGELKQDLLHAEINGHKSKIPVSREGDDFTLFLPSGSYHFRALQTEVVEEQA
NSEDKLKAPMNGTIVTHLVEVGDEVEAGQGLMVMEAMKMEYTIEAPFDGTVAAFFFQPGE
LVSDGALLLEVTAKEDA