Protein Info for Shew_2411 in Shewanella loihica PV-4

Annotation: FAD dependent oxidoreductase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 686 TIGR03197: tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein MnmC, C-terminal domain" amino acids 273 to 683 (411 residues), 452.8 bits, see alignment E=5.9e-140 PF01266: DAO" amino acids 274 to 653 (380 residues), 140.9 bits, see alignment E=3.8e-45

Best Hits

Swiss-Prot: 100% identical to MNMC_SHELP: tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC (mnmC) from Shewanella loihica (strain ATCC BAA-1088 / PV-4)

KEGG orthology group: None (inferred from 100% identity to slo:Shew_2411)

Predicted SEED Role

"tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase (EC 2.1.1.61)" (EC 2.1.1.61)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.61

Use Curated BLAST to search for 2.1.1.61

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QFM9 at UniProt or InterPro

Protein Sequence (686 amino acids)

>Shew_2411 FAD dependent oxidoreductase (RefSeq) (Shewanella loihica PV-4)
MPNIPLRVNSLATEHPNNAQNSDKMPTFDAIFSHLSAIASHNSHQIIALLPSSDANWPAA
LIAERLTQAGSKQHLQKQHLHLHLFAQHQASWLKALAESETLASPAKEQIKAICDARVSG
SHRLKLVNARLIIDIHLGDPLTQLKDLVSPSLASQAIQGWLANTQATDEALIWQMARLSQ
DNAEFLLLENNDVNLDKTSNNANTNLLTQLIIKAGFTCYRLNLSLKDDQLVTLAEKPSLA
SLEIAMVERRALRRQQLDKFAFNPLTQGREGETAIIGGGVASANLALSLAERGKKVSFFC
MDKAPGEQASGNKQGAIYPLLTPEHGSLSHYFLLGYLFSRQRIKQLLESGHEIPHDFCGV
LQTGHDERSHKRLTKIINAQPWAESIARPVDALQATALAGVTIEHQGIYYPLAGWVSPQA
FTRAAISQAERLGKQTSHYQCQITAIRFENQQWYLSAIQDGQKVEFGPYANLVLANGRHL
TDFAQTDHLPISGFRGQVSHIPERAPLKDLKTVLCAHGYLTPAHDKLHCTGASYVKDASN
LDYSAVEQVENLDKIRTSYGGEWTKAVDITGHSARVGVRMVTRDHAPMMGCAPDFEAISA
TYISHQQTKKSTKESAKCWQTTSAPVHQGLFILGGLGSRGLTSGPLAAEILAAQLCGELL
PATQDILALLNPNRMWMRKLIKGKAL