Protein Info for Shew_2407 in Shewanella loihica PV-4

Annotation: putative periplasmic protease (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 343 transmembrane" amino acids 12 to 33 (22 residues), see Phobius details amino acids 179 to 202 (24 residues), see Phobius details PF08496: Peptidase_S49_N" amino acids 2 to 153 (152 residues), 191.2 bits, see alignment E=1e-60 PF01343: Peptidase_S49" amino acids 156 to 305 (150 residues), 163.8 bits, see alignment E=3e-52

Best Hits

Swiss-Prot: 58% identical to SOHB_ECOLI: Probable protease SohB (sohB) from Escherichia coli (strain K12)

KEGG orthology group: K04774, serine protease SohB [EC: 3.4.21.-] (inferred from 100% identity to slo:Shew_2407)

Predicted SEED Role

"Possible protease sohB (EC 3.4.21.-)" (EC 3.4.21.-)

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.-

Use Curated BLAST to search for 3.4.21.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QFM5 at UniProt or InterPro

Protein Sequence (343 amino acids)

>Shew_2407 putative periplasmic protease (RefSeq) (Shewanella loihica PV-4)
MEFLYEYGLFLAKAVTIVVAIVAVVVLVLASAVKQKADKGELKLTNVSEELKDLKHDLKE
ELLSKKQFKAYEKGLKAEAKAKEKAEKADGEAAPTPRVFVIDFKGSIDAHEVASLREEIS
AILAIAEPGDEVVVNVESGGGMVHGYGLAASQLDRLRSAEIPLTICVDKVAASGGYMMAC
VANTIYAAPFAIVGSIGVVAQIPNFNRLLKKHDIDYEQHTAGDFKRTLTLFGENTDEGRQ
KFQEELEETHVLFKAFISKYRPTLDIAKVATGEHWYGQQALDLGLVDGLSTSDDVVMKLA
EEKTVIKVRYQLKKKIADKLAHSASLAVNAVFNRLAEKNQPLN