Protein Info for Shew_2334 in Shewanella loihica PV-4

Name: secF
Annotation: preprotein translocase subunit SecF (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 315 transmembrane" amino acids 13 to 37 (25 residues), see Phobius details amino acids 137 to 155 (19 residues), see Phobius details amino acids 162 to 183 (22 residues), see Phobius details amino acids 189 to 211 (23 residues), see Phobius details amino acids 240 to 258 (19 residues), see Phobius details amino acids 265 to 289 (25 residues), see Phobius details PF07549: Sec_GG" amino acids 34 to 60 (27 residues), 43.7 bits, see alignment (E = 1.3e-15) TIGR00966: protein-export membrane protein SecF" amino acids 39 to 284 (246 residues), 285.1 bits, see alignment E=5.6e-89 TIGR00916: protein-export membrane protein, SecD/SecF family" amino acids 101 to 281 (181 residues), 194.1 bits, see alignment E=2.8e-61 PF02355: SecD_SecF" amino acids 109 to 291 (183 residues), 237.4 bits, see alignment E=8.6e-75

Best Hits

Swiss-Prot: 49% identical to SECF_PSEAE: Protein translocase subunit SecF (secF) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K03074, preprotein translocase subunit SecF (inferred from 100% identity to slo:Shew_2334)

Predicted SEED Role

"Protein-export membrane protein SecF (TC 3.A.5.1.1)" (TC 3.A.5.1.1)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QFF2 at UniProt or InterPro

Protein Sequence (315 amino acids)

>Shew_2334 preprotein translocase subunit SecF (RefSeq) (Shewanella loihica PV-4)
MFEILSIKGTINFLRHAVAISALSLILVIGSLASLAVNGINWGLDFTGGTVVEMEFKNPV
DLNQLRAALTSDETDGAVVQNFGSSRDVLIRLPVKEGVKSDLQVAHVMEAAAKVDPGVSQ
KRVEFVGPQVGKELAEQGGLAVLVALICILIYVSFRFEWRLALGSVAALAHDVIITLGVF
SLFQLEFDLTVLAGVLTVVGYSLNDTIVVFDRIRENFLKMRKGTPEEVVNNSITQTMSRT
IITTGTTLVVVVALFLKGGTMIHGFATALLMGIFVGTFSSIYVASFLAVKLGINREHMMP
VEIEKEGADQDALMP