Protein Info for Shew_1993 in Shewanella loihica PV-4

Annotation: fumarate/nitrate reduction transcriptional regulator (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 249 PF00027: cNMP_binding" amino acids 50 to 131 (82 residues), 54.4 bits, see alignment E=1.5e-18 PF13545: HTH_Crp_2" amino acids 167 to 240 (74 residues), 63.2 bits, see alignment E=2.6e-21 PF00325: Crp" amino acids 192 to 223 (32 residues), 55.2 bits, see alignment 6.9e-19

Best Hits

Swiss-Prot: 90% identical to ETRA_SHEON: Electron transport regulator A (etrA) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K01420, CRP/FNR family transcriptional regulator, anaerobic regulatory protein (inferred from 100% identity to slo:Shew_1993)

Predicted SEED Role

"Fumarate and nitrate reduction regulatory protein" in subsystem Oxidative stress

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QEG1 at UniProt or InterPro

Protein Sequence (249 amino acids)

>Shew_1993 fumarate/nitrate reduction transcriptional regulator (RefSeq) (Shewanella loihica PV-4)
MTDNIKSRRSAVPGCAIHCHDCSMGTLCIPFTLNNNELDQLDDIIERKKPIQKGEQIFKS
GDSLKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDVIGFDGIHSQIHQSFAQALETSMV
CEIPFDTLDELSGTMPKLRQQIMRLMSNEIQSDQEMILLLSKKNAEERLAAFISNLASRF
GSRGFSPKEFRLTMTRGDIGNYLGLTVETISRLLGRFQKAELIEVKGKYITIVDFDSLNK
LAGNNKIGR