Protein Info for Shew_1942 in Shewanella loihica PV-4

Annotation: NADH:flavin oxidoreductase/NADH oxidase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 671 PF00724: Oxidored_FMN" amino acids 6 to 327 (322 residues), 271.8 bits, see alignment E=2e-84 PF07992: Pyr_redox_2" amino acids 376 to 653 (278 residues), 81 bits, see alignment E=2.1e-26 PF13450: NAD_binding_8" amino acids 379 to 433 (55 residues), 30.4 bits, see alignment 7.9e-11

Best Hits

Swiss-Prot: 56% identical to FADH_ECOLI: 2,4-dienoyl-CoA reductase (fadH) from Escherichia coli (strain K12)

KEGG orthology group: K00219, 2,4-dienoyl-CoA reductase (NADPH2) [EC: 1.3.1.34] (inferred from 100% identity to slo:Shew_1942)

MetaCyc: 56% identical to 2,4-dienoyl-CoA reductase (Escherichia coli K-12 substr. MG1655)
2,4-dienoyl-CoA reductase (NADPH). [EC: 1.3.1.34]

Predicted SEED Role

"2,4-dienoyl-CoA reductase [NADPH] (EC 1.3.1.34)" (EC 1.3.1.34)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.3.1.34

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QEB0 at UniProt or InterPro

Protein Sequence (671 amino acids)

>Shew_1942 NADH:flavin oxidoreductase/NADH oxidase (RefSeq) (Shewanella loihica PV-4)
MSFPHLLEPLDLGFTQLKNRVLMGSMHTGLEEEKGGFEKLAKFYQERAAGGVGLIVTGGI
SPNLRGRLAPNACQLSFPWQVAKHKVVTSAVHEAGGKICMQILHAGRYGYHPFSQAPSKI
KSPITPFTPSAMSARQVRSTIKDYATTAALAKKAGYDGVEVMGSEGYLINQFICARTNKR
NDEWGGEFSQRAKFPVEIVKAIRERVGSDFIIVFRLSMLDLVDNGSSWEEVVQLAKWLES
AGVTIINTGIGWHEARVPTIATSVPRGAFAWVTERLKQEVSVPLIATNRINTPEIGEQII
SSGQADMVSMARPFLADAEFVNKAAAGESQLINTCIGCNQACLDHTFALKRATCLVNPRA
CYETELNFVPATSKKRIAVMGAGPAGMAFSIYAASRGHQVVLFEAKDEVGGQFNLARKIP
GKEEFDETIRYFTEQMKLNKVELRLNTRLDASVVRDEPFDEVVIAAGVVPRAIDLPGFDS
PKVVNYQQVLNGEVEVGERVALIGAGGIGFDMAHYLGEQESTTLKPDTWLKQWGIDKAYG
ERGGLTTPQPEHTSRKIYLLQRKTSKMGKGLGKTTGWIHRAVAKHHDVEMLNGVSYQKFD
ELGLHIKVGEEAQVLEVDNVVLCAGQESNRSLVDEMKNTGLPVHLIGGVDVAAELDAKRA
IRQGAELAASI