Protein Info for Shew_1925 in Shewanella loihica PV-4
Annotation: 3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) (RefSeq)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 50% identical to ODBA_CAEEL: 2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial (bckd-1A) from Caenorhabditis elegans
KEGG orthology group: K00166, 2-oxoisovalerate dehydrogenase E1 component, alpha subunit [EC: 1.2.4.4] (inferred from 100% identity to slo:Shew_1925)Predicted SEED Role
"Branched-chain alpha-keto acid dehydrogenase, E1 component, alpha subunit (EC 1.2.4.4)" in subsystem Isoleucine degradation or Leucine Degradation and HMG-CoA Metabolism or Valine degradation (EC 1.2.4.4)
MetaCyc Pathways
- 2-oxoisovalerate decarboxylation to isobutanoyl-CoA (3/3 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.2.4.4
Use Curated BLAST to search for 1.2.4.4
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A3QE93 at UniProt or InterPro
Protein Sequence (392 amino acids)
>Shew_1925 3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) (RefSeq) (Shewanella loihica PV-4) MSNATSNTETVHRVSFLDKDSLSIPILKILQADGTTFENAVLPVIDQDLAAKIYDTCVFT RVLDERMLAAQRQGRISFYMTCTGEEAAVVGSVAALDQDDVILAQYREHAAIRYRGFTTE QFMNQMFSNEKDLGKGRQMPIHYGCEALNYQTISSPLATQIPQATGVGYSLKMQGKRNVA ICYFGEGAASEGDFHAGLNMAAVLNSPVIFFCRNNGYAISTPTNEQFAGNGIASRGVGYG MHTIRVDGNDMLAVMAATQQARAYALEHNKPVLIEAMTYRLGAHSSSDDPSGYRSKDEEA KWQQHDPVKRFKLWLINKGWLAESDDAALYEKYREEVLAAVKVAEKLPAPKIDEIIEDVL DEPTPRLKQQLTELKQHIKKYPDSYPRSAGRD