Protein Info for Shew_1889 in Shewanella loihica PV-4

Annotation: alpha amylase, catalytic region (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 541 PF00128: Alpha-amylase" amino acids 14 to 465 (452 residues), 439.4 bits, see alignment E=3e-135 PF16657: Malt_amylase_C" amino acids 465 to 508 (44 residues), 29.3 bits, see alignment 1.1e-10

Best Hits

KEGG orthology group: K01187, alpha-glucosidase [EC: 3.2.1.20] (inferred from 100% identity to slo:Shew_1889)

Predicted SEED Role

"Maltodextrin glucosidase (EC 3.2.1.20)" in subsystem Maltose and Maltodextrin Utilization (EC 3.2.1.20)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.20

Use Curated BLAST to search for 3.2.1.20

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QE57 at UniProt or InterPro

Protein Sequence (541 amino acids)

>Shew_1889 alpha amylase, catalytic region (RefSeq) (Shewanella loihica PV-4)
MNQLSWWRGAVIYQIYPRSLMDSNGDGVGDLQGIISKLDYIASLNVDAIWISPFFKSPMK
DFGYDISDYRAIDPLFGTMADFDELIDKAHGLGIKVIIDQVLSHTSDQHAWFSESRQSRD
NAKQDWYVWADPNPDGTAPNNWLAIFGGCAWEWEPRRQQYYLHNFLTSQPDLNFHNPDVR
QAVLDNVKFWLDKGVDGFRLDAITFCYHDEQLRDNPPKPEDKRQGRGFSEDNPYAYQYHY
YNNTRPQTVGFIEELRALINQYPGVVTLGEVSSEDSLATMAEYTQGDDRLHMAYSFELLT
DDFSPAYIRHTVEALEQSIGDGWPCWAIGNHDVQRVATRWGRDAFNSDMAKMLNAMLCSL
RGSVCSYQGEELGLGEVEIAYEQLQDPFGITFWPMFKGRDGCRTPMPWRHDANHAGFSDN
TPWLPVPADHKAVAVDVQEANPESILNHYRHMLAWRKLHPILVTGDIKFIESTDALLVFE
RRLGEQTLLVAFNLSGQPQSFSLAGLGDKALSPLEGHGLPKAELKGDLVQFDGFGCFYCW
K