Protein Info for Shew_1865 in Shewanella loihica PV-4

Annotation: phosphoenolpyruvate-protein phosphotransferase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 568 signal peptide" amino acids 1 to 18 (18 residues), see Phobius details PF05524: PEP-utilisers_N" amino acids 4 to 125 (122 residues), 75.4 bits, see alignment E=6.5e-25 TIGR01417: phosphoenolpyruvate-protein phosphotransferase" amino acids 4 to 563 (560 residues), 488.1 bits, see alignment E=1.6e-150 PF00391: PEP-utilizers" amino acids 151 to 224 (74 residues), 62.8 bits, see alignment E=2.9e-21 PF02896: PEP-utilizers_C" amino acids 255 to 537 (283 residues), 315.2 bits, see alignment E=6.1e-98

Best Hits

Swiss-Prot: 39% identical to PT1_SALTY: Phosphoenolpyruvate-protein phosphotransferase (ptsI) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K08483, phosphotransferase system, enzyme I, PtsI [EC: 2.7.3.9] (inferred from 100% identity to slo:Shew_1865)

MetaCyc: 39% identical to PTS enzyme I (Escherichia coli K-12 substr. MG1655)
Phosphoenolpyruvate--protein phosphotransferase. [EC: 2.7.3.9]

Predicted SEED Role

"Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9)" in subsystem Fructose and Mannose Inducible PTS or Fructose utilization or Mannitol Utilization (EC 2.7.3.9)

Isozymes

Compare fitness of predicted isozymes for: 2.7.3.9

Use Curated BLAST to search for 2.7.3.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QE33 at UniProt or InterPro

Protein Sequence (568 amino acids)

>Shew_1865 phosphoenolpyruvate-protein phosphotransferase (RefSeq) (Shewanella loihica PV-4)
MTMRGIAVSAGIAFGQAKVLRTYESKLDYHLLPPSQLAREQQRLNRALKTLIKQSQACAA
KLDPESDNYQLIEADLLLLEDEELLAELSDTIGKRQFSAALAVEHSFAKQAQAMQEADSP
YLARRAEDVLSLGQRLIRTLLTGHCDNLSRLPENAIVLAKDITPAEFATLPLERVSALVL
QTGGVTSHTAILARSAGIPTLMSCPWQTLEVTDGMVLAVDAINGELYLEPDETQIDDLNA
QKQQADERKSALLALKGTVTQTKDGRSIPLLANVGCISEINHLADVGAEGVGLFRTEFLF
MNNHELPDENRQYQLYCDALQLLDGKPLTIRSMDLGADKEVPTLAMDSEENPALGLRGVR
YTLAHPKLFSAQLKAILRAANHGPIRLMFPMVNQVEELEAVLALLELCKQELVEAEKGFG
ELELGIVVETPAAVFNLASMLPLLDFVSIGTNDLTQYTMAADRANPLLIDQYPVLSPVII
RLIAQIIEQAKAAKVRVSMCGELASNPSATALLIGLGLEEFSVNLASLLEVKQALSRWSY
PDCVELAHKALQISRIDALNQLLTHCHS