Protein Info for Shew_1864 in Shewanella loihica PV-4
Annotation: glucose-specific PTS system component (RefSeq)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 61% identical to PTGA_SALTY: PTS system glucose-specific EIIA component (crr) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
KEGG orthology group: K02777, PTS system, glucose-specific IIA component [EC: 2.7.1.69] (inferred from 100% identity to slo:Shew_1864)MetaCyc: 60% identical to Enzyme IIAGlc (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-168 [EC: 2.7.1.201]; TRANS-RXN-157 [EC: 2.7.1.201, 2.7.1.199]; RXN0-17 [EC: 2.7.1.201, 2.7.1.199, 2.7.1.192]
Predicted SEED Role
"PTS system, glucose-specific IIA component"
MetaCyc Pathways
- superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (22/26 steps found)
- superpathway of glycolysis and the Entner-Doudoroff pathway (15/17 steps found)
- glycolysis I (from glucose 6-phosphate) (11/13 steps found)
KEGG Metabolic Maps
- Aminosugars metabolism
- Ascorbate and aldarate metabolism
- Fructose and mannose metabolism
- Galactose metabolism
- Glycolysis / Gluconeogenesis
- Nucleotide sugars metabolism
- Starch and sucrose metabolism
Isozymes
Compare fitness of predicted isozymes for: 2.7.1.69
Use Curated BLAST to search for 2.7.1.192 or 2.7.1.199 or 2.7.1.201 or 2.7.1.69
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A3QE32 at UniProt or InterPro
Protein Sequence (169 amino acids)
>Shew_1864 glucose-specific PTS system component (RefSeq) (Shewanella loihica PV-4) MGFLSRIRRLISGQPPITGGIDVYAPVSGEIVAIEKVPDVVFAEKIVGDGIAIAPKGKQI VAPVDGTIGKIFETNHAFSIESPQGLELFVHFGVGTVELRGNGFTRLAEEGQQVKAGDPI LEFDLDYLKAHVESLLTPVVLANMEDIQGLDKRHGSIEAGKDVIFSVQL