Protein Info for Shew_1833 in Shewanella loihica PV-4

Annotation: putative serine protein kinase, PrkA (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 644 PF08298: AAA_PrkA" amino acids 20 to 380 (361 residues), 595.2 bits, see alignment E=4.5e-183 PF06798: PrkA" amino acids 382 to 636 (255 residues), 240.1 bits, see alignment E=3.2e-75

Best Hits

Swiss-Prot: 85% identical to YEAG_ECOL6: Uncharacterized protein YeaG (yeaG) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: None (inferred from 85% identity to eae:EAE_21735)

MetaCyc: 85% identical to protein kinase YeaG (Escherichia coli K-12 substr. MG1655)
2.7.11.d,2.7.11.11,2.7.12.1,2.7.11.12,2.7.11.17,2.7.11.1,2.7.11.13,2.7.11.22,2.7.11.21,2.7.11.34,2.7.11.31 [EC: 2.7.11.1, 2.7.11.11, 2.7.11.12, 2.7.11.13, 2.7.11.17, 2.7.11.21, 2.7.11.22, 2.7.11.31, 2.7.11.34, 2.7.11.d, 2.7.12.1]

Predicted SEED Role

"Serine protein kinase (prkA protein), P-loop containing"

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.11.1

Use Curated BLAST to search for 2.7.11.1 or 2.7.11.11 or 2.7.11.12 or 2.7.11.13 or 2.7.11.17 or 2.7.11.21 or 2.7.11.22 or 2.7.11.31 or 2.7.11.34 or 2.7.11.d or 2.7.12.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QE01 at UniProt or InterPro

Protein Sequence (644 amino acids)

>Shew_1833 putative serine protein kinase, PrkA (RefSeq) (Shewanella loihica PV-4)
MGIFEHYQQRYEKKLDEEYSLQEFLAICKEDRIAYASAAERLLTAIGKPEVIDTSKDPIL
SRIFSNRLISRYPAFKDFYGMEDSIEQIVAYLKHSAQGLEESKQILYLLGPVGGGKSSLA
EKLKSLMQRVPIYVLSANGQRSPVNDHPFCLFNPEEDGELLKNEYNIPERYLKTIMSPWA
VKRLHEFGGDISQFKVVKVFPAILDQIGIAKTEPGDENNQDISSLVGKVDIRQLEHYAQH
DADAYSYSGALCRANQGLMEFVEMFKAPIKVLHPLLTATQEGNYNGTEGLSALPFNGIIL
AHSNESEWTSFRNNKTNEAFLDRVYIVKVPYCLRVSEEIQIYEKLLTNSELAQAPCAPYT
LETLAQFSVLSRIKAPENSSIYSKMRVYDGESLKDTDPKAKSYQEYRDYAGVDEGMQGLS
TRFAFKILSKVFNFDHSEIAANPVHLFYVLERQIEQEQFPAETAERYMEFLKGYLTPKYI
EFIGKEIQTAYLESYSEYGQNIFDRYVTYADFWIQDQEYRDPETGQLFDRAALNAELEKI
EKPAGISNPKDFRNEIVNFVLRARANNEGSNPLWTSYEKLRTVIEKKMFSNTEDLLPVIS
FNTKTSTDDQRKHDDFVNRMMEKGYTKKQVRLLSEWYLRVRKSS