Protein Info for Shew_1777 in Shewanella loihica PV-4

Annotation: Bcr/CflA subfamily drug resistance transporter (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 407 transmembrane" amino acids 20 to 42 (23 residues), see Phobius details amino acids 62 to 81 (20 residues), see Phobius details amino acids 89 to 108 (20 residues), see Phobius details amino acids 114 to 135 (22 residues), see Phobius details amino acids 147 to 168 (22 residues), see Phobius details amino acids 174 to 194 (21 residues), see Phobius details amino acids 228 to 249 (22 residues), see Phobius details amino acids 260 to 279 (20 residues), see Phobius details amino acids 291 to 313 (23 residues), see Phobius details amino acids 319 to 342 (24 residues), see Phobius details amino acids 353 to 375 (23 residues), see Phobius details amino acids 381 to 400 (20 residues), see Phobius details TIGR00710: drug resistance transporter, Bcr/CflA subfamily" amino acids 23 to 393 (371 residues), 232.2 bits, see alignment E=6.7e-73 PF07690: MFS_1" amino acids 29 to 369 (341 residues), 146.2 bits, see alignment E=1.2e-46 PF06609: TRI12" amino acids 64 to 208 (145 residues), 28.4 bits, see alignment E=6.1e-11

Best Hits

KEGG orthology group: K07552, MFS transporter, DHA1 family, bicyclomycin/chloramphenicol resistance protein (inferred from 100% identity to slo:Shew_1777)

Predicted SEED Role

"Multidrug resistance transporter, Bcr/CflA family" in subsystem Copper homeostasis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QDU5 at UniProt or InterPro

Protein Sequence (407 amino acids)

>Shew_1777 Bcr/CflA subfamily drug resistance transporter (RefSeq) (Shewanella loihica PV-4)
MNKASIPSSELSQPQGIPNLALLIPMLSAIVAITPLAIDMYLPAMSTLASSFNSSVTTVQ
QSLSLYLAGYALGMLTFGPIADRIGRRPLVLLGLTGFGLISLALAFVTSVEVFLGLRFLQ
AFIGAAATVVVPGYIKEVYGKNTAKGMSYVSLIMMLAPLIAPTMGSLILELGEWHLIFFI
LACYASLLLVLVLTKLRMPSDAQTSGRSQRSFFGAYAVVFTKPGVKLHIASGVMTSFAFF
CYLTASPFVYMEVFGLDKSLFAILFSTNVGALMLANVINSRIVVKYGSRRMLKVATLLAA
VAGIGLLSVNLFGLSYHFTVAALIPFMGFLGVMSVNADAIVLMKFQQETGTATAVIGTLR
FGFGALAGPLLALFYTGTAVPFSALMLGAVLLVGLCQFLAHSQQRQG