Protein Info for Shew_1600 in Shewanella loihica PV-4

Annotation: putative glycerol-3-phosphate acyltransferase PlsX (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 100% identical to PLSX_SHELP: Phosphate acyltransferase (plsX) from Shewanella loihica (strain ATCC BAA-1088 / PV-4)

KEGG orthology group: K03621, glycerol-3-phosphate acyltransferase PlsX [EC: 2.3.1.15] (inferred from 100% identity to slo:Shew_1600)

Predicted SEED Role

"Phosphate:acyl-ACP acyltransferase PlsX" in subsystem Glycerolipid and Glycerophospholipid Metabolism in Bacteria

MetaCyc Pathways

phosphatidylglycerol biosynthesis I (6/6 steps found)
phosphatidylglycerol biosynthesis II (6/6 steps found)
superpathway of phospholipid biosynthesis III (E. coli) (10/12 steps found)
CDP-diacylglycerol biosynthesis I (4/4 steps found)
CDP-diacylglycerol biosynthesis II (4/4 steps found)
oleate biosynthesis III (cyanobacteria) (2/3 steps found)
superpathway of cardiolipin biosynthesis (bacteria) (9/13 steps found)
phosphatidate biosynthesis (yeast) (3/5 steps found)
diacylglycerol and triacylglycerol biosynthesis (3/7 steps found)
palmitoyl ethanolamide biosynthesis (2/6 steps found)
superpathway of stearidonate biosynthesis (cyanobacteria) (2/6 steps found)
stigma estolide biosynthesis (2/7 steps found)
anandamide biosynthesis II (2/8 steps found)
anandamide biosynthesis I (4/12 steps found)
superpathway of phospholipid biosynthesis II (plants) (10/28 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.15

Use Curated BLAST to search for 2.3.1.15

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QDB9 at UniProt or InterPro

Protein Sequence (342 amino acids)

>Shew_1600 putative glycerol-3-phosphate acyltransferase PlsX (RefSeq) (Shewanella loihica PV-4)
MTNLTLALDAMGGDFGPRITVPATLQALRLYPFLHVIMVGDRSQIEPLLSHLESDLLSRI
ELVHTTEVVKMCDRPVHALRNCKQSSMRLAIELVRDKKAQACLSAGNTGALMAISKVLLK
TLPGIDRPALVSCLPAVNHKPVYLLDLGANVSCCSETLFQFAVMGSVLCEAVDKNPAPKV
ALLNVGIEEIKGNDQVQQAGQLLQQSPQLNYAGFIEGDDIYSGNVDVIVCDGFVGNITLK
TSEGIARLLVHQLKKGLTQGFFVRLLAKLIAPRINSVLNQMNPDHYNGASLIGLRGIVVK
SHGSADESAYLQAISLAVTEAQRRLPKMIEERLESILLDINN