Protein Info for Shew_1583 in Shewanella loihica PV-4

Name: yebU
Annotation: rRNA (cytosine-C(5)-)-methyltransferase RsmF (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 486 PF17125: Methyltr_RsmF_N" amino acids 4 to 96 (93 residues), 81.6 bits, see alignment E=1.2e-26 PF22458: RsmF-B_ferredox" amino acids 8 to 72 (65 residues), 27.2 bits, see alignment E=8.5e-10 TIGR00446: NOL1/NOP2/sun family putative RNA methylase" amino acids 37 to 307 (271 residues), 309.9 bits, see alignment E=6.2e-97 PF01189: Methyltr_RsmB-F" amino acids 117 to 305 (189 residues), 155 bits, see alignment E=5.3e-49 PF21150: YebU_pre-PUA_dom" amino acids 326 to 403 (78 residues), 79.4 bits, see alignment E=3.7e-26 PF13636: Methyltranf_PUA" amino acids 417 to 466 (50 residues), 49.1 bits, see alignment 1.2e-16

Best Hits

Swiss-Prot: 100% identical to RSMF_SHELP: Ribosomal RNA small subunit methyltransferase F (rsmF) from Shewanella loihica (strain ATCC BAA-1088 / PV-4)

KEGG orthology group: K11392, ribosomal RNA small subunit methyltransferase F [EC: 2.1.1.-] (inferred from 100% identity to slo:Shew_1583)

Predicted SEED Role

No annotation

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.-

Use Curated BLAST to search for 2.1.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QDA2 at UniProt or InterPro

Protein Sequence (486 amino acids)

>Shew_1583 rRNA (cytosine-C(5)-)-methyltransferase RsmF (RefSeq) (Shewanella loihica PV-4)
MVQLNQNFINSIAKDMPDHLSMDEFISYCAKPLRPSIRVNTLKITTADFITMMNAKGWQF
EPVPWCEDGFWVKVDDSVQLGNTVEHIQGLFYIQEASSMLPPKALFPEPIEDATSLTLLD
MASAPGSKTTQLAAMMNNQGLLVANEYSSSRVKVLHANVQRMGASNLALTHFDARVFGKY
LFESFDAILLDAPCGGEGTVRKDPDALKHWDESEIEAIASVQRDLIESAFLALKPGGSLV
YSTCTLNRRENQDVCLHLKATYGDAVQFESLAGLFPGAEKASTSEGFLHVWPQIFDSEGF
FIAKLTKIAAVPRNSEEPRKQKNFPFTPIDKKSRQALCDYFDTSFGIKLDGQGILMQRDD
EFWLFPTNVDPLIGKIRFQRIGLKLADALKKGFKPRHEAVMALADRLRGIPLDEAQAIQY
LMGRDIALTSKEKPQGERLVSYQGAHLGLVKHLGNKLKNSLPRDLVRDKICSSSAMAVDQ
QATGED