Protein Info for Shew_1511 in Shewanella loihica PV-4

Annotation: UMP phosphatase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00702: Hydrolase
  • amino acids 5 to 216 (212 residues), 51.9 bits, E=2.6e-17
• TIGR01460: HAD hydrolase, family IIA
  • amino acids 8 to 225 (218 residues), 184.9 bits, E=2.3e-58
• PF13344: Hydrolase_6
  • amino acids 8 to 107 (100 residues), 79.6 bits, E=3.2e-26
• PF13419: HAD_2
  • amino acids 166 to 222 (57 residues), 24.3 bits, E=6.5e-09
• PF13242: Hydrolase_like
  • amino acids 176 to 244 (69 residues), 73.8 bits, E=1.7e-24

Best Hits

Swiss-Prot: 73% identical to NAGD_ECO57: Ribonucleotide monophosphatase NagD (nagD) from Escherichia coli O157:H7

KEGG orthology group: K02566, NagD protein (inferred from 100% identity to slo:Shew_1511)

MetaCyc: 73% identical to UMP phosphatase (Escherichia coli K-12 substr. MG1655)
5'-nucleotidase. [EC: 3.1.3.5]

Predicted SEED Role

"Phosphatase NagD predicted to act in N-acetylglucosamine utilization subsystem" in subsystem Chitin and N-acetylglucosamine utilization

MetaCyc Pathways

• inosine 5'-phosphate degradation (3/4 steps found)
• purine nucleotides degradation II (aerobic) (8/11 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• NAD salvage pathway III (to nicotinamide riboside) (2/3 steps found)
• adenosine nucleotides degradation II (3/5 steps found)
• adenosine nucleotides degradation I (5/8 steps found)
• guanosine nucleotides degradation I (2/4 steps found)
• guanosine nucleotides degradation II (2/4 steps found)
• guanosine nucleotides degradation III (2/4 steps found)
• superpathway of guanosine nucleotides degradation (plants) (3/6 steps found)
• purine nucleotides degradation I (plants) (7/12 steps found)
• ureide biosynthesis (3/7 steps found)
• tunicamycin biosynthesis (2/9 steps found)
• NAD salvage (plants) (3/11 steps found)
• superpathway of purines degradation in plants (7/18 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Nicotinate and nicotinamide metabolism
• Purine metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.5

Use Curated BLAST to search for 3.1.3.5

Sequence Analysis Tools

See A3QD30 at UniProt or InterPro

Protein Sequence (252 amino acids)

>Shew_1511 UMP phosphatase (RefSeq) (Shewanella loihica PV-4)
MRPKMKNVICDIDGVLLHDNKLIPGSDKFIHRILEQGNPLVILTNYPVQTGKDLQNRLGA
AGIQVPEECFYTSAMATADFLKHQTGDKAFVIGEGALTHELYKAGFTITDINPDFVIVGE
TRSYNWDMIHKAAQFVAAGARFIATNPDTHGPAHSPACGALCAPIERISGRMPFYVGKPS
SWIIRSALNHIGGHSENTVIIGDNMRTDILAGFQAGLETILVTSGVSQIKDIDKEPFRPN
HVFDCAGDIDVI