Protein Info for Shew_1464 in Shewanella loihica PV-4

Name: nadE
Annotation: NAD synthetase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00552: NAD+ synthetase
  • amino acids 28 to 284 (257 residues), 210.7 bits, E=1.1e-66
• PF02540: NAD_synthase
  • amino acids 32 to 281 (250 residues), 210.5 bits, E=1.1e-66

Best Hits

Swiss-Prot: 79% identical to NADE_SHESH: NH(3)-dependent NAD(+) synthetase (nadE) from Shewanella sediminis (strain HAW-EB3)

KEGG orthology group: K01916, NAD+ synthase [EC: 6.3.1.5] (inferred from 100% identity to slo:Shew_1464)

Predicted SEED Role

"NAD synthetase (EC 6.3.1.5)" in subsystem NAD and NADP cofactor biosynthesis global or NAD regulation (EC 6.3.1.5)

MetaCyc Pathways

• aspartate superpathway (24/25 steps found)
• NAD de novo biosynthesis I (5/6 steps found)
• NAD de novo biosynthesis IV (anaerobic) (5/6 steps found)
• NAD de novo biosynthesis III (4/6 steps found)
• NAD salvage pathway II (PNC IV cycle) (3/5 steps found)
• NAD salvage pathway I (PNC VI cycle) (3/7 steps found)

KEGG Metabolic Maps

• Nicotinate and nicotinamide metabolism
• Nitrogen metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.1.5

Sequence Analysis Tools

See A3QCY3 at UniProt or InterPro

Protein Sequence (288 amino acids)

>Shew_1464 NAD synthetase (RefSeq) (Shewanella loihica PV-4)
MLTVNLSGVGPVKGQIIREMKVQPHIEVEYEVQRRIAFIKAKLKEARATSLVLGISGGVD
SSLAGRLCQLAVDELNSEGEYEGSYQFIAVRLPFKVQKDEDEAQMACQFIQPSKLVTVNI
GEGVEGIHHQTLAGLEVAGVISHPHSNVDFVKGNVKARMRMIAQYEIAGLTGGLVVGTDH
SAENITGFYTKWGDGACDLAPLFGLSKRQVRQLAAALGAPSVLVDKAPTADLECDKPQLE
DEVALGLTYDQIDDFLEGKPVDAAVEARLIAIYNATQHKRKPIPTLYD