Protein Info for Shew_1443 in Shewanella loihica PV-4

Annotation: integral membrane sensor signal transduction histidine kinase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 607 transmembrane" amino acids 12 to 34 (23 residues), see Phobius details amino acids 319 to 339 (21 residues), see Phobius details PF00512: HisKA" amino acids 392 to 456 (65 residues), 30.5 bits, see alignment E=3.2e-11 PF02518: HATPase_c" amino acids 498 to 603 (106 residues), 89.1 bits, see alignment E=2.7e-29

Best Hits

KEGG orthology group: K10125, two-component system, NtrC family, C4-dicarboxylate transport sensor histidine kinase DctB [EC: 2.7.13.3] (inferred from 100% identity to slo:Shew_1443)

Predicted SEED Role

"Signal transduction histidine kinase regulating C4-dicarboxylate transport system"

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QCW2 at UniProt or InterPro

Protein Sequence (607 amino acids)

>Shew_1443 integral membrane sensor signal transduction histidine kinase (RefSeq) (Shewanella loihica PV-4)
MPYMSKIITRRKYLGVGFIILLGLAFTLRVAYWFHLEHGYEMTRQQSNAQMKELLSFLSG
SLSRYESIPHVLSTNPLLASALLKQDDPDTITPLNRYLEEIQSITEALDIYLVNIDGDAI
AASNWQQSYSFIGKNYAFRPYFKDAIEGKMGRYFAVGTSSNKRGYYFSYPIYSHGYPVTP
SQGEAKVLGAIVVKVDVTEIEQQSTGMARGSNYEFAISDPDHIIFLSSRPEWRLTALTPV
THQLKQRIESSRRYANREISPLAIEPPFAPSLAADYQIYQIGDDKRGAEFMDNAAYMSDA
GWQVHILSPMTPIYRSLPPLLLLYGALYLLCALALLFAIEKRKNTRRMQQAHEQLEQRVK
ERTQALETSHQQLKEAQDELIQAAKLTVIGSLSASINHEINQPLAAIRSYAQNTQTLLSR
AKWDDVSSNIMTIIELTDRLAAIVSQFKSFTRKSQGNDKAISVAQCIRDALTIVQPEVDK
QGIDLVLNNTECQTLLWGDPIRLQQVLINLMSNAMVAMRANNPKQLIIGVVVSNRLEITI
EDTGTGIDESQMQKIFDPYYTSSRQGLGLGLSISRRIVEAMQGKISVANRQQGGAIFQIT
LPIHAVG