Protein Info for Shew_1439 in Shewanella loihica PV-4

Annotation: maleylacetoacetate isomerase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02798: GST_N
  • amino acids 1 to 78 (78 residues), 49.1 bits, E=1.7e-16
• TIGR01262: maleylacetoacetate isomerase
  • amino acids 3 to 213 (211 residues), 291.7 bits, E=1.5e-91
• PF13417: GST_N_3
  • amino acids 6 to 82 (77 residues), 55.7 bits, E=1.5e-18
• PF13409: GST_N_2
  • amino acids 9 to 79 (71 residues), 52.2 bits, E=2.2e-17
• PF14497: GST_C_3
  • amino acids 127 to 199 (73 residues), 30.9 bits, E=7.9e-11
• PF00043: GST_C
  • amino acids 142 to 199 (58 residues), 26.2 bits, E=2.3e-09

Best Hits

Swiss-Prot: 54% identical to MAAI_VIBCH: Probable maleylacetoacetate isomerase (maiA) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K01800, maleylacetoacetate isomerase [EC: 5.2.1.2] (inferred from 100% identity to slo:Shew_1439)

MetaCyc: 48% identical to glutathion-dependent maleylpyruvate isomerase (Ralstonia sp. U2)
Maleylpyruvate isomerase. [EC: 5.2.1.4]

Predicted SEED Role

"Maleylacetoacetate isomerase (EC 5.2.1.2) @ Glutathione S-transferase, zeta (EC 2.5.1.18)" (EC 2.5.1.18, EC 5.2.1.2)

MetaCyc Pathways

• L-tyrosine degradation I (4/5 steps found)
• gentisate degradation I (1/3 steps found)
• 4-hydroxy-2-nonenal detoxification (1/4 steps found)
• 5-nitroanthranilate degradation (2/6 steps found)
• glutathione-mediated detoxification I (3/8 steps found)
• 3-phenylpropanoate degradation (4/10 steps found)
• pentachlorophenol degradation (3/10 steps found)
• gliotoxin biosynthesis (1/9 steps found)
• glutathione-mediated detoxification II (1/9 steps found)
• indole glucosinolate activation (intact plant cell) (3/12 steps found)
• camalexin biosynthesis (2/12 steps found)

KEGG Metabolic Maps

• Drug metabolism - cytochrome P450
• Glutathione metabolism
• Metabolism of xenobiotics by cytochrome P450
• Styrene degradation
• Tyrosine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.5.1.18, 5.2.1.2

Use Curated BLAST to search for 2.5.1.18 or 5.2.1.2 or 5.2.1.4

Sequence Analysis Tools

See A3QCV8 at UniProt or InterPro

Protein Sequence (214 amino acids)

>Shew_1439 maleylacetoacetate isomerase (RefSeq) (Shewanella loihica PV-4)
MKLLGYWRSSAAYRVRIALNLKGLDAELESVHLVKNGGEQHLPEYAALNPQELVPTLIEG
DNEFVLSQSLAIIEYLDEQYGGAMMLPTAPKARAEVRAMALSIACEVHPLNNLKVLQYLT
NTLELDEDAKNAWYHHWIHQGFSAFEKQLEKHAGSYCYGDEVTLADLCLIPQVYNANRFK
VDLSTYPNIRRIWDNCHQLEAFVLAAPERQADAS