Protein Info for Shew_1168 in Shewanella loihica PV-4

Annotation: glycogen branching enzyme (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 765 PF22019: GlgB_N" amino acids 19 to 130 (112 residues), 54.9 bits, see alignment E=1.5e-18 TIGR01515: 1,4-alpha-glucan branching enzyme" amino acids 141 to 756 (616 residues), 877.3 bits, see alignment E=2.9e-268 PF02922: CBM_48" amino acids 156 to 237 (82 residues), 70.8 bits, see alignment E=2e-23 PF02806: Alpha-amylase_C" amino acids 662 to 758 (97 residues), 87.6 bits, see alignment E=1.2e-28

Best Hits

Swiss-Prot: 69% identical to GLGB_SHESM: 1,4-alpha-glucan branching enzyme GlgB (glgB) from Shewanella sp. (strain MR-4)

KEGG orthology group: K00700, 1,4-alpha-glucan branching enzyme [EC: 2.4.1.18] (inferred from 100% identity to slo:Shew_1168)

Predicted SEED Role

"1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18)" in subsystem Glycogen metabolism or Trehalose Biosynthesis (EC 2.4.1.18)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QC40 at UniProt or InterPro

Protein Sequence (765 amino acids)

>Shew_1168 glycogen branching enzyme (RefSeq) (Shewanella loihica PV-4)
MTKQIPVQTPAQTPLIAPSEIEALLAADYVDIFALLGMHAIEMAPATKSSKGIKEAKAVS
LVKRLVVRCFLPGAERVELIAKQDGRKVAELDKCHDEGIFAGLAGRRINPFEYRLRVHYP
FSVEEINDPYQYASLLSDEALYLFSEGRQEQAYRFMGANWREVDGVSGVQFCVWAPNAKR
VSLVHDAIDWDGRRQVMRHHPANGVWEIFIPSMKALTHYKYEIVTQEGERLLKADPFGRA
MQGAPGNASIVPPQLDYAWQDKRWLAKRAKQSWHDQPISIYEVHLGSWRRQGENGEQYLS
YSQLIDQLIPYVVEMGYTHLQLMPLCEYPFDGSWGYQPVGLFAPTHRFGQPHELKAFVDA
CHQAGIGVILDWVAAHFPKDPHGLTRFDGSCLYEHEDPRQGEHPDWDTLIYNYGRGEVQS
YLLSNAHYWLQEFHLDGLRLDAVSSMLYLDYSREPHQWLPNAHGGRENLDAIAFLQDLNT
RLYQGFPGIMMIAEESTAWPGVTKRVDEGGLGFGFKWNMGWMNDSLNYIGRDPIHRSHHH
NELTFSLMYCFTEQFILSLSHDEVVHGKGSLLHKIPGDDWQKFATLRAYLGFMWGHPGKK
LLFMGADFAQRDEWDHQHSLDWHLLAFEPHQGMQRWVKDLNSLYRQSVALYGDDHHGEGF
SWLDCDNASASVLSFIRQSGDKQLIFIVNMTPEVYREFRIGLPQPGRYHERLNSDSHYYG
GSDVGNQGVIHTEPEAYQGQAQSACISVPPLACLVLELDESVSCD