Protein Info for Shew_1099 in Shewanella loihica PV-4

Annotation: isoleucyl-tRNA synthetase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 940 TIGR00392: isoleucine--tRNA ligase" amino acids 14 to 846 (833 residues), 983.6 bits, see alignment E=4.5e-300 PF00133: tRNA-synt_1" amino acids 28 to 643 (616 residues), 519.3 bits, see alignment E=2e-159 PF09334: tRNA-synt_1g" amino acids 58 to 201 (144 residues), 40.3 bits, see alignment E=5.3e-14 PF13603: tRNA-synt_1_2" amino acids 279 to 361 (83 residues), 28.6 bits, see alignment E=3e-10 PF08264: Anticodon_1" amino acids 688 to 845 (158 residues), 105.4 bits, see alignment E=7.9e-34 PF06827: zf-FPG_IleRS" amino acids 902 to 928 (27 residues), 32.4 bits, see alignment (E = 2e-11)

Best Hits

Swiss-Prot: 71% identical to SYI_AERHH: Isoleucine--tRNA ligase (ileS) from Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / DSM 30187 / JCM 1027 / KCTC 2358 / NCIMB 9240)

KEGG orthology group: K01870, isoleucyl-tRNA synthetase [EC: 6.1.1.5] (inferred from 71% identity to aha:AHA_0682)

MetaCyc: 71% identical to isoleucine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Isoleucine--tRNA ligase. [EC: 6.1.1.5]; 6.1.1.5 [EC: 6.1.1.5]; 3.1.1.- [EC: 6.1.1.5]; RXN-23924 [EC: 6.1.1.5]

Predicted SEED Role

"Isoleucyl-tRNA synthetase (EC 6.1.1.5)" (EC 6.1.1.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QBX2 at UniProt or InterPro

Protein Sequence (940 amino acids)

>Shew_1099 isoleucyl-tRNA synthetase (RefSeq) (Shewanella loihica PV-4)
MSDYKSTLNLPETEFPMRGNLAQREPEMLKSWTEKKLYQKIRESRTGAKPFILHDGPPYA
NGSIHIGHSVNKILKDIIIKSKTLSGFDAPYIPGWDCHGLPIELKVEQKVGKPGQKVSAA
EFRQKCREYAAAQVDGQREDFIRLGVLGDWQNPYLTMNFDTEANIVRSLAKVIDNGHLHK
GVKPVHWCTDCGSALAEAEVEYEDKTSPAIDVAFVAVDQAAVLAKFGLEQSDAEVATVIW
TTTPWTLPANRGLSMAGDIDYDLVEFTKDGSKRAIIVAQELVESCIERYGAELHSVLGTV
KGQALELLRFKHPFYDFDVPVIIGDHVTVDSGTGVVHTAPGHGQDDFVIGQKYGLEVANP
VGDNGVYKPDTEFFAGQHVFKANDNVVALLEEKGALLNHVAYRHSYPHCWRHKTPIIFRA
TPQWFISMDQKGLRTQSLSEIEKTQWIPDWGQSRIEKMVENRPDWCISRQRTWGVPITLF
VHRDTEELHPDSVALMERVAHRIEQEGIQAWWDLDAAELLGEEADQYRKVTDTLDVWYDS
GSTFSSVVAARPEYNGHPVDLYLEGSDQHRGWFMSSLMISTAMNGKAPYKQVLTHGFTVD
GQGRKMSKSIGNVIAPLQVTNKLGADILRLWVAATDYSGEMTVSDEILKRSADAYRRIRN
TARFLLANLNGFDPVNDTVAIEDMVALDRWVVRRAAALQAEILEAYEQYNFHLVTQKLMQ
FCSVELGSFYLDIIKDRQYTAKRDSLARRSCQSALFLIAEAMVRWIAPILSFTADEVWQS
LPGEREEFVFTQTWFEGLVSVTLDSDLSDDYWANLITVRDEVNKAIEQARREQRVGGSLE
AEVTIYADEALTESLALLGDELRFVLLTSNTQIAPLANAGDDAVETELAGLKLLVDKTEA
AKCDRCWHHREDVGQVEAHPLLCTRCVTNIEGDGEVRQFA