Protein Info for Shew_1083 in Shewanella loihica PV-4

Annotation: apurinic endonuclease Apn1 (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 283 TIGR00587: apurinic endonuclease (APN1)" amino acids 7 to 279 (273 residues), 352.5 bits, see alignment E=7.7e-110 PF01261: AP_endonuc_2" amino acids 23 to 280 (258 residues), 218.9 bits, see alignment E=4.7e-69

Best Hits

Swiss-Prot: 70% identical to END4_PROMH: Probable endonuclease 4 (nfo) from Proteus mirabilis (strain HI4320)

KEGG orthology group: K01151, deoxyribonuclease IV [EC: 3.1.21.2] (inferred from 100% identity to slo:Shew_1083)

MetaCyc: 67% identical to endonuclease IV (Escherichia coli K-12 substr. MG1655)
3.1.4.-; 3.1.4.-; 3.1.3.-; Deoxyribonuclease IV (phage-T(4)-induced). [EC: 3.1.21.2]; 3.1.21.- [EC: 3.1.21.2]

Predicted SEED Role

"Endonuclease IV (EC 3.1.21.2)" in subsystem DNA repair, bacterial (EC 3.1.21.2)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.21.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QBV6 at UniProt or InterPro

Protein Sequence (283 amino acids)

>Shew_1083 apurinic endonuclease Apn1 (RefSeq) (Shewanella loihica PV-4)
MPDTHWVGAHVSAAGGIANAPLNAQKIGANAFALFTKNQRRWQSAPLCPSQIDAFKANCE
QANIHPDAILPHDSYLINLGHPDEEQLARSREAFLDEMQRCEQMGLTLLNFHPGSHLNKI
GIDECLARISESINLALDKTQGVSAIIENTAGQGSNLGHSFEQLAQIIDKVEDKSRVGVC
IDTCHMFAAGYDLTTLEACEESFGQFDAIVGNHYLKAMHLNDSKTPLNSRVDRHHSLGAG
YIGMDAFSYIMKHPATRQIPLILETIEPEIWPQEINWLRSQTR