Protein Info for Shew_1068 in Shewanella loihica PV-4

Name: tyrA
Annotation: bifunctional chorismate mutase/prephenate dehydrogenase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01799: chorismate mutase
  • amino acids 14 to 96 (83 residues), 144.5 bits, E=4.1e-47
• PF01817: CM_2
  • amino acids 17 to 94 (78 residues), 78.7 bits, E=4.9e-26
• PF02153: PDH_N
  • amino acids 151 to 228 (78 residues), 39.1 bits, E=8.2e-14
• PF20463: PDH_C
  • amino acids 252 to 354 (103 residues), 43 bits, E=7.4e-15

Best Hits

Swiss-Prot: 80% identical to TYRA_SHEON: T-protein (tyrA) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K14187, chorismate mutase / prephenate dehydrogenase [EC: 1.3.1.12 5.4.99.5] (inferred from 100% identity to slo:Shew_1068)

MetaCyc: 58% identical to fused chorismate mutase/prephenate dehydrogenase (Escherichia coli K-12 substr. MG1655)
Chorismate mutase. [EC: 5.4.99.5]; Prephenate dehydrogenase. [EC: 5.4.99.5, 1.3.1.12]

Predicted SEED Role

"Chorismate mutase I (EC 5.4.99.5) / Cyclohexadienyl dehydrogenase (EC 1.3.1.12)(EC 1.3.1.43)" in subsystem Chorismate Synthesis or Phenylalanine and Tyrosine Branches from Chorismate (EC 1.3.1.12, EC 1.3.1.43, EC 5.4.99.5)

MetaCyc Pathways

• superpathway of chorismate metabolism (51/59 steps found)
• superpathway of aromatic amino acid biosynthesis (18/18 steps found)
• superpathway of L-phenylalanine biosynthesis (10/10 steps found)
• superpathway of L-tyrosine biosynthesis (10/10 steps found)
• L-phenylalanine biosynthesis I (3/3 steps found)
• L-tyrosine biosynthesis I (3/3 steps found)
• L-tyrosine biosynthesis III (2/4 steps found)
• 4-hydroxy-3-prenylbenzoate biosynthesis (1/4 steps found)
• L-phenylalanine biosynthesis II (1/4 steps found)
• L-tyrosine biosynthesis II (1/4 steps found)
• superpathway of L-phenylalanine and L-tyrosine biosynthesis (1/5 steps found)
• bacilysin biosynthesis (1/10 steps found)
• salinosporamide A biosynthesis (3/15 steps found)
• superpathway of novobiocin biosynthesis (4/19 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of phenylpropanoids
• Novobiocin biosynthesis
• Phenylalanine, tyrosine and tryptophan biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 5.4.99.5

Use Curated BLAST to search for 1.3.1.12 or 1.3.1.43 or 5.4.99.5

Sequence Analysis Tools

See A3QBU1 at UniProt or InterPro

Protein Sequence (384 amino acids)

>Shew_1068 bifunctional chorismate mutase/prephenate dehydrogenase (RefSeq) (Shewanella loihica PV-4)
MEHAQMSEKTTNELEKLREHIDSVDQQLLHLLRKRLDLVAQVGGVKHAAGVPIYAPQREA
SMLAKRREEAAKMNVSPQLIEDILRRLMRESYLNEKDVGFKQVKTDLGHVVIVGGQGKLG
GLFAQMLSLSGYQVKSLDKDDWQQADAIFDGAGMVIVTVPISITCELIRDKLTSLPSSCI
LADLTSIKEKPLEAMLASHQGPVVGLHPMFGPDVGSLAKQVVVVCHGRGREQYEWLLEQI
GIWGARLVEADAQQHDKAMQLVQAMRHFSSFVYGLNLYREAADIDNLLQFSSPIYRLELA
MVGRLFAQSPELYADIIFAQQESLTAIGDYLDNYAQALELLKRGDRQGFIREFEEVAAWF
GDFAPQFQRESRAMLQSVNDMKTG