Protein Info for Shew_1008 in Shewanella loihica PV-4

Annotation: metal dependent phosphohydrolase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 413 PF01743: PolyA_pol" amino acids 3 to 122 (120 residues), 71 bits, see alignment E=1.8e-23 PF12627: PolyA_pol_RNAbd" amino acids 149 to 212 (64 residues), 52.2 bits, see alignment E=6.1e-18 PF01966: HD" amino acids 229 to 321 (93 residues), 38.1 bits, see alignment E=2.5e-13

Best Hits

Swiss-Prot: 100% identical to CCA_SHELP: Multifunctional CCA protein (cca) from Shewanella loihica (strain ATCC BAA-1088 / PV-4)

KEGG orthology group: K00974, tRNA nucleotidyltransferase (CCA-adding enzyme) [EC: 2.7.7.72 3.1.3.- 3.1.4.-] (inferred from 100% identity to slo:Shew_1008)

MetaCyc: 62% identical to fused CCA tRNA nucleotidyltransferase/phosphohydrolase (Escherichia coli K-12 substr. MG1655)
TRNA-CYTIDYLYLTRANSFERASE-RXN [EC: 2.7.7.72]

Predicted SEED Role

"tRNA nucleotidyltransferase (EC 2.7.7.21) (EC 2.7.7.25)" in subsystem Polyadenylation bacterial or tRNA nucleotidyltransferase (EC 2.7.7.21, EC 2.7.7.25)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.-, 3.1.4.-

Use Curated BLAST to search for 2.7.7.21 or 2.7.7.25 or 2.7.7.72 or 3.1.3.- or 3.1.4.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QBN1 at UniProt or InterPro

Protein Sequence (413 amino acids)

>Shew_1008 metal dependent phosphohydrolase (RefSeq) (Shewanella loihica PV-4)
MKIYLVGGAVRDQLLNIPIKDRDYMVVGATVQEMLDKGYRQVGKDFPVFLHPKTQQEYAL
ARTERKTGVGYGGFSVYAAPDVTLEEDLLRRDLTINAIAQDETGRVFDPYGGQADIEQRL
LRHVSDAFVEDPLRVLRVARFAARFQPLGFKVAPETMALMQRIAASGELEALTPERVFQE
LDKALSTEAPQVFFEVLREAGGLAILFPEIEALFGIPQPEQWHPEIDTGIHTLMVLEQAA
RLSQDKQVRFAALVHDLGKALSPKEHLPKHHGHGQKGLPLIRALCERFRVPNDYRDLALL
VSDQHQNIHNAFELRAETMVKLFDKADLWRKPERLPQLLLACEADCKGRTGLKERPYPQG
AYVQHCFELARNVAIKPIIEAGFKGAEIKAQLHKQRVEVIDQYKRKTAVNAKP