Protein Info for Shew_0973 in Shewanella loihica PV-4

Annotation: extracellular solute-binding protein (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 366 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details PF01547: SBP_bac_1" amino acids 37 to 300 (264 residues), 70.2 bits, see alignment E=6.2e-23 PF13416: SBP_bac_8" amino acids 44 to 330 (287 residues), 110 bits, see alignment E=3.9e-35 PF13343: SBP_bac_6" amino acids 76 to 309 (234 residues), 71.3 bits, see alignment E=1.8e-23

Best Hits

Swiss-Prot: 61% identical to SPUE_PSEAE: Spermidine-binding periplasmic protein SpuE (spuE) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K11073, putrescine transport system substrate-binding protein (inferred from 100% identity to slo:Shew_0973)

MetaCyc: 54% identical to putrescine ABC transporter periplasmic binding protein (Escherichia coli K-12 substr. MG1655)
ABC-25-RXN [EC: 7.6.2.11, 7.6.2.16]

Predicted SEED Role

"Putrescine ABC transporter putrescine-binding protein PotF (TC 3.A.1.11.2)" in subsystem Polyamine Metabolism (TC 3.A.1.11.2)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.6.2.11 or 7.6.2.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QBJ6 at UniProt or InterPro

Protein Sequence (366 amino acids)

>Shew_0973 extracellular solute-binding protein (RefSeq) (Shewanella loihica PV-4)
MKLLKKMTTLALVTAGVFTSSVVSAEEVVRVYNWSDYIAEDTLANFEKETGIKVIYDVFD
SNEVVEAKLLSGRSGYDIVVPSNNFLAKQIKAGAFQKLDQSQLTNHKNLNPELMKQLETA
DPRNEHAVPYLWGTTGIGYNEAKVKQVLGEDAPVDSLELIFNPKYAEKIAKCGFSLLDSA
DEMLPMAMIYLGLDPNSTKSEDYAKAGEVMAKIRPFVTYFHSSRYITDLANGDICVAFGY
SGDVFQAAARAEEAENGNVIGYAIPKEGSNLWFDMLAIPADAKNVKNAHTFINYLLRPEV
IAPITNYVAYANPNVPAQELVDEEIRNDAAIYPTPEVLSRLYVADVRPMKAQRAMTRVWT
KVKSGM