Protein Info for Shew_0972 in Shewanella loihica PV-4

Annotation: glutamate--ammonia ligase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00120: Gln-synt_C
  • amino acids 111 to 443 (333 residues), 355.8 bits, E=1.3e-110

Best Hits

KEGG orthology group: K01915, glutamine synthetase [EC: 6.3.1.2] (inferred from 100% identity to slo:Shew_0972)

Predicted SEED Role

"Gamma-glutamyl-putrescine synthetase (EC 6.3.1.11)" (EC 6.3.1.11)

MetaCyc Pathways

• L-arginine biosynthesis II (acetyl cycle) (9/10 steps found)
• L-glutamate and L-glutamine biosynthesis (6/7 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• superpathway of ammonia assimilation (plants) (3/3 steps found)
• ammonia assimilation cycle I (2/2 steps found)
• ammonia assimilation cycle II (2/2 steps found)
• L-glutamine biosynthesis I (1/1 steps found)
• superpathway of ornithine degradation (6/8 steps found)
• putrescine degradation II (3/4 steps found)
• superpathway of L-arginine and L-ornithine degradation (9/13 steps found)
• superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation (7/11 steps found)
• L-aspartate degradation II (aerobic) (1/3 steps found)
• L-aspartate degradation III (anaerobic) (1/3 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Glutamate metabolism
• Nitrogen metabolism
• Urea cycle and metabolism of amino groups

Isozymes

Compare fitness of predicted isozymes for: 6.3.1.2

Use Curated BLAST to search for 6.3.1.11 or 6.3.1.2

Sequence Analysis Tools

See A3QBJ5 at UniProt or InterPro

Protein Sequence (449 amino acids)

>Shew_0972 glutamate--ammonia ligase (RefSeq) (Shewanella loihica PV-4)
MKKLIDYLKANKITEVECVISDMTGIARGKIAPVDKFIDEKGMRLPESVLLQTVTGDYVD
DDLYDSLLDKADIDFVCVPDENAVFMLPWTVEATAQVIHDTFDKMGNPIELSPRNLLKKV
LKLYEDKGWKPVIAPEMEFYLTHRNSDPDMPLIPPVGRSGRKESGRQSFSIDAANEYDPL
FEDMYDWCEAQGLDIDTLIHEEGTAQMEINFSHGDALSLADQVFVFKRTLREAALKHDVC
ATFMAKPVTDEPGSAMHIHQSVVDIKTGKNIFTNEDGTKSAKFLCYIGGLQKYIPELLPL
MAPNVNSFRRFLPGTSAPVNLEWGDENRTCGLRIPESSPQNRRIENRIAGADANSYLAIA
ATLLCGYMGMVEDVKPSTPVQGRANENRQGISLPLTLEEALAVMSESSAVREYLGDTFTS
GYIAVKQADLENFRRVISSWEREFLLLTV