Protein Info for Shew_0932 in Shewanella loihica PV-4

Annotation: radical SAM domain-containing protein (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 362 TIGR04337: AmmeMemoRadiSam system radical SAM enzyme" amino acids 11 to 359 (349 residues), 652.7 bits, see alignment E=5.5e-201 PF04055: Radical_SAM" amino acids 86 to 237 (152 residues), 53.3 bits, see alignment E=1.9e-18

Best Hits

KEGG orthology group: K04069, pyruvate formate lyase activating enzyme [EC: 1.97.1.4] (inferred from 100% identity to slo:Shew_0932)

Predicted SEED Role

"COG1180: Radical SAM, Pyruvate-formate lyase-activating enzyme like"

Isozymes

Compare fitness of predicted isozymes for: 1.97.1.4

Use Curated BLAST to search for 1.97.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QBF5 at UniProt or InterPro

Protein Sequence (362 amino acids)

>Shew_0932 radical SAM domain-containing protein (RefSeq) (Shewanella loihica PV-4)
MLTAPPAYFPTQYWHALDDGRVCCDVCPRKCTLREGKRGACFVRMHHQGEIVLTSYGRSS
GFCIDPIEKKPLNHFYPGSSVLSFGTAGCNLSCLFCQNWDMSKSRQMDTLCDSAMPDELA
ATARRHGCKSIAFTYNDPVVFMEYAIDAAQAAHELGLKSVAVSAGYICEAPRIEFYRYMD
AANIDLKAFSESFYHKICHGQLQPVLETLLYLKHETQVWFEITTLLIPGENDSDEELNAM
CQWLVENLGPDVPLHFSAFHPDFKLLDRPPTPLSTLQRARRIALNHGLHYVYCGNVHDPD
SDATYCASCHKRLIERDWYQLGQYALDEQGTCRYCGAPLPGRFARVREDFGARRIAVRIH
RD