Protein Info for Shew_0851 in Shewanella loihica PV-4

Annotation: phospho-2-dehydro-3-deoxyheptonate aldolase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 TIGR00034: 3-deoxy-7-phosphoheptulonate synthase" amino acids 6 to 349 (344 residues), 566.9 bits, see alignment E=5.9e-175 PF00793: DAHP_synth_1" amino acids 45 to 338 (294 residues), 332.4 bits, see alignment E=7.5e-104

Best Hits

Swiss-Prot: 75% identical to AROG_ECO57: Phospho-2-dehydro-3-deoxyheptonate aldolase, Phe-sensitive (aroG) from Escherichia coli O157:H7

KEGG orthology group: K01626, 3-deoxy-7-phosphoheptulonate synthase [EC: 2.5.1.54] (inferred from 100% identity to slo:Shew_0851)

MetaCyc: 75% identical to 3-deoxy-7-phosphoheptulonate synthase, Phe-sensitive (Escherichia coli K-12 substr. MG1655)
3-deoxy-7-phosphoheptulonate synthase. [EC: 2.5.1.54]

Predicted SEED Role

"2-keto-3-deoxy-D-arabino-heptulosonate-7-phosphate synthase I alpha (EC 2.5.1.54)" in subsystem Chorismate Synthesis or Common Pathway For Synthesis of Aromatic Compounds (DAHP synthase to chorismate) (EC 2.5.1.54)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.5.1.54

Use Curated BLAST to search for 2.5.1.54

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QB75 at UniProt or InterPro

Protein Sequence (350 amino acids)

>Shew_0851 phospho-2-dehydro-3-deoxyheptonate aldolase (RefSeq) (Shewanella loihica PV-4)
MYYQTDDVRINKIKELLPPVAILERFPASENASATVFNARQNIHNILKKEDDRLLVIIGP
CSIHDPKAALEYGERLAKLREQYKDQLEVVMRVYFEKPRTTVGWKGLINDPYMDNSFKLN
DGLRTARKLLLDLNELGVPTAGEFLDMITPQYVADLMCWGAIGARTTESQVHRELASGLS
CPVGFKNGTDGTIKVAIDAIGAASAPHHFLSVTKYGHSAIVSTKGNPDCHIILRGGKEPN
YSAEHVQAISEQLAKANLPQNIMVDFSHANSSKQFKRQMLVGEDVASQVSAGERAIFGVM
VESHLVEGRQDYIEGQPLCYGQSITDACIGWEDTETLLANLNRCVLERRS