Protein Info for Shew_0732 in Shewanella loihica PV-4

Annotation: diguanylate cyclase with PAS/PAC sensor (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 446 transmembrane" amino acids 6 to 31 (26 residues), see Phobius details PF00989: PAS" amino acids 155 to 256 (102 residues), 30.3 bits, see alignment E=8.9e-11 TIGR00229: PAS domain S-box protein" amino acids 165 to 265 (101 residues), 44 bits, see alignment E=2.2e-15 PF08447: PAS_3" amino acids 166 to 253 (88 residues), 88 bits, see alignment E=1e-28 PF13426: PAS_9" amino acids 167 to 258 (92 residues), 22.2 bits, see alignment E=3.4e-08 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 266 to 431 (166 residues), 136.3 bits, see alignment E=8.1e-44 PF00990: GGDEF" amino acids 270 to 429 (160 residues), 150.3 bits, see alignment E=1e-47

Best Hits

KEGG orthology group: None (inferred from 100% identity to slo:Shew_0732)

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QAV6 at UniProt or InterPro

Protein Sequence (446 amino acids)

>Shew_0732 diguanylate cyclase with PAS/PAC sensor (RefSeq) (Shewanella loihica PV-4)
MAEMSLPLLLIIFLAIGLIAVATCMLALRFILRRRGVDFSALENSIEDGLLLIDEQAKIL
AHNTQAAALMKAEGSDFTGQALSLWLKDSSGEQLQVFSDRQLGTSLNLGHEILSFTRLAR
EPRGLLLLVRHHNLEQDIRQDIARYHHSQYFAKIGTWDWQVGTDTLYWSDAIFGIFGYKL
GEVTPSYEFFYSRVHPEDRDKVKAGEARCIATGENHDEEYRILWPDGSVHWVRETGNLVK
DSDGQLLKMVGVVRDITQEKALHHKLEKMAHQDPLTGLPNRLQLELRLSEAIREASQSGG
RLGLIFIDLNQFKAINDSLGHQVGDRVLMTAATRLQSLKGSEDLVARIGGDEFVMLLPQW
PADRPLEEEVARLAEALFERFAEPVIQTGRGIAASIGFAIYPDHAGHMDALLHVADQAMY
IAKGRGDNQYHLGPDHRMDLGHRLAR