Protein Info for Shew_0710 in Shewanella loihica PV-4

Name: metH
Annotation: B12-dependent methionine synthase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02082: methionine synthase
  • amino acids 21 to 1206 (1186 residues), 1758.3 bits, E=0
• PF02574: S-methyl_trans
  • amino acids 26 to 333 (308 residues), 340 bits, E=4.2e-105
• PF00809: Pterin_bind
  • amino acids 368 to 606 (239 residues), 211.1 bits, E=5.1e-66
• PF02607: B12-binding_2
  • amino acids 672 to 744 (73 residues), 85.4 bits, 6.1e-28
• PF02310: B12-binding
  • amino acids 759 to 848 (90 residues), 66.1 bits, 6.9e-22
• PF02965: Met_synt_B12
  • amino acids 951 to 1222 (272 residues), 430.6 bits, E=6.4e-133

Best Hits

KEGG orthology group: K00548, 5-methyltetrahydrofolate--homocysteine methyltransferase [EC: 2.1.1.13] (inferred from 67% identity to aha:AHA_0432)

Predicted SEED Role

"5-methyltetrahydrofolate--homocysteine methyltransferase (EC 2.1.1.13)" in subsystem Methionine Biosynthesis (EC 2.1.1.13)

MetaCyc Pathways

• aspartate superpathway (24/25 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (18/18 steps found)
• folate transformations II (plants) (11/11 steps found)
• folate transformations III (E. coli) (9/9 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (9/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (9/9 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (11/12 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (8/8 steps found)
• L-methionine biosynthesis I (5/5 steps found)
• folate transformations I (10/13 steps found)
• L-methionine biosynthesis III (3/4 steps found)
• L-methionine salvage from L-homocysteine (1/3 steps found)
• superpathway of L-methionine salvage and degradation (5/16 steps found)

KEGG Metabolic Maps

• Methionine metabolism
• One carbon pool by folate

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.13

Sequence Analysis Tools

See A3QAT4 at UniProt or InterPro

Protein Sequence (1240 amino acids)

>Shew_0710 B12-dependent methionine synthase (RefSeq) (Shewanella loihica PV-4)
MATQSPVRNVRQINDAIRARLNKEILILDGAMGTMIQNHKLEEADFRGERFKQWPCDLKG
NNDLLVLTQPELIKQIHRDYLLAGADIIETNTFNATQVAMADYEMQELSREINLEGARLA
RTACDEVAAESGRQCYVAGVLGPTNRTCSISPDVNDPGYRNIHFDDLVEAYIESTQALIE
GGADIIMVETIFDTLNAKAALFAIETLFDQLGQRLPVMISGTITDASGRTLTGQTTEAFY
NSLRHVKPLSIGLNCALGPQELRPYVEELSKIAECYVSAHPNAGLPNEFGGYDETPEQMA
EVIGPWAEEGLLNIIGGCCGTTPDHIKAIRAAVIKHGARELPDLPVACRLSGLEPLTIDA
DSLFVNVGERTNVTGSAKFLRLIKTGEYEEALSVARDQVENGAQIIDINMDEGMLDGVEV
MQKFLNLIASEPDISRVPIMIDSSKWEVIEAGLKCIQGKGVVNSISLKEGEAKFIEQATL
VKRYGAAAIIMAFDEVGQADTMARKIEICTRAYRVLVDKVGFPPEDIIFDPNIFAIATGI
DEHDNYAVDFIEATREIKRTLPHAMISGGVSNVSFSFRGNNPVREAIHAVFLYHAIQAGM
DMGIVNAGQLAIYDDIDPDLKARVEAVVQNLPCPVQGSNNTEQLLEIAEQYRGDGAAGAK
KEDLEWRSWPVNKRLEHALVKGITEFIDQDTEEARQLASRPLDVIEGPLMDGMNVVGDLF
GSGKMFLPQVVKSARVMKKAVAYLNPYIEAEKVEGESNGKILMVTVKGDVHDIGKNIVGV
VLACNGYEVVDLGVMVPVEKIIEVAKAEKVDIIGMSGLITPSLDEMVHNVKAFHKAGLNI
PSIIGGATCSKIHTAVKIAPHAPEGAIYIADASRAVPMVAKLINKETRQATIDAAYEEYR
VMRDKRNSQAKRKQIVSLEAARENRCQQDWNAHTPFVPNQLGRQVFDDYPLEDLVDRIDW
TPFFRSWELHGHFPKILDDEVVGEEARKLYQDAKAMLKTIIDEKWLTAKAVIGLFPANTV
NYDDIEIYTDESRSKVEMTTHHLRMQIERVGNDNFCLADFVAPKDSGVADYMGGFAVTAG
HGIDEHVARFEANHDDYSAIMLKCLADRLVEAFAERMHERVRKEFWGYAADENLDNEALI
REKYKGIRPAPGYPACPDHTEKGLLWELLKPDETIDLKITESYAMFPTAAVSGWYFAHPQ
SRYFGVTNIGRDQVEDYAKRKGMSVEETERWLAPVLDYDV