Protein Info for Shew_0667 in Shewanella loihica PV-4

Annotation: phosphoadenosine phosphosulfate reductase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02057: phosphoadenosine phosphosulfate reductase
  • amino acids 26 to 247 (222 residues), 281.7 bits, E=5e-88
• TIGR00434: phosophoadenylyl-sulfate reductase
  • amino acids 39 to 247 (209 residues), 273.4 bits, E=1.5e-85
• PF01507: PAPS_reduct
  • amino acids 53 to 225 (173 residues), 191 bits, E=9.4e-61

Best Hits

Swiss-Prot: 84% identical to CYSH_SHEON: Phosphoadenosine phosphosulfate reductase (cysH) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K00390, phosphoadenosine phosphosulfate reductase [EC: 1.8.4.8] (inferred from 100% identity to slo:Shew_0667)

MetaCyc: 64% identical to phosphoadenosine phosphosulfate reductase (Escherichia coli K-12 substr. MG1655)
Phosphoadenylyl-sulfate reductase (thioredoxin). [EC: 1.8.4.8]

Predicted SEED Role

"Phosphoadenylyl-sulfate reductase [thioredoxin] (EC 1.8.4.8)" in subsystem Cysteine Biosynthesis (EC 1.8.4.8)

MetaCyc Pathways

• superpathway of sulfate assimilation and cysteine biosynthesis (9/9 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (11/12 steps found)
• assimilatory sulfate reduction I (4/4 steps found)
• assimilatory sulfate reduction III (3/3 steps found)
• assimilatory sulfate reduction IV (3/4 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (7/10 steps found)

KEGG Metabolic Maps

• Sulfur metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.8.4.8

Sequence Analysis Tools

See A3QAP1 at UniProt or InterPro

Protein Sequence (250 amino acids)

>Shew_0667 phosphoadenosine phosphosulfate reductase (RefSeq) (Shewanella loihica PV-4)
MDNSVISAQALQALLSAPKAEQDAELARVNAFLAGLTPAERVIWGLAYLPGTHALSSSFG
IQGAVMLHLVTQVQQDIPVILTDTGYLFPETYHFIDELTERLKLNLKVYRAPMTSAWQEA
RFGRLWEQGLDGLEQYNRLNKVTPMQTALKELEVGTWFAGLRRSQSSTREDLPILAIHGD
RYKLLPILEWSNKDVHEYLTKHDLPYHPLWEQGYVSVGDTHSSRPLELGMTEEETRFNGL
KRECGLHYEI