Protein Info for Shew_0628 in Shewanella loihica PV-4

Annotation: (glutamate--ammonia-ligase) adenylyltransferase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 956 PF03710: GlnE" amino acids 41 to 276 (236 residues), 296.4 bits, see alignment E=1.7e-92 amino acids 558 to 814 (257 residues), 295.7 bits, see alignment E=2.7e-92 PF08335: GlnD_UR_UTase" amino acids 307 to 444 (138 residues), 89.8 bits, see alignment E=1.7e-29 amino acids 852 to 927 (76 residues), 35 bits, see alignment E=1.4e-12

Best Hits

Swiss-Prot: 100% identical to GLNE_SHELP: Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme (glnE) from Shewanella loihica (strain ATCC BAA-1088 / PV-4)

KEGG orthology group: K00982, glutamate-ammonia-ligase adenylyltransferase [EC: 2.7.7.42] (inferred from 100% identity to slo:Shew_0628)

Predicted SEED Role

"Glutamate-ammonia-ligase adenylyltransferase (EC 2.7.7.42)" in subsystem Ammonia assimilation (EC 2.7.7.42)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.42

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QAK2 at UniProt or InterPro

Protein Sequence (956 amino acids)

>Shew_0628 (glutamate--ammonia-ligase) adenylyltransferase (RefSeq) (Shewanella loihica PV-4)
MENMSEQALPHEVNQVAEQHWQRLAEAWPDISEQLTPEQTKELKTIFGLSDFVAEQLCRH
PNWIVSLFEGQLSMLARDSFSSELHSILAGANDEEQVKAILRRYRNRQMVRLAWRDFLGY
AELNDSLLDLSALAEALIIAARDWLYGQMCQQYGTPCDSEGNPQPLMILGMGKLGGRELN
FSSDIDLIFTFPEHGETQGGRRSQDNQQFFIRMGQRLVNLLNQVTVDGFVYRVDMRLRPY
GESGPLVVSFSGLEDYYQEQGRDWERYAMVKARALGPWSAYSDELHDMLRPFVYRRYIDF
SAIESLRKMKQLITQEVRRRRLTDNIKLGAGGIREVEFVVQSFQLIRGGREPALRQQSLF
GAIDTLYKLGQLEYLAVDELKQSYLMLRRVENLLQAIGDQQTQTLPQHLLDWQRLCFALG
MAGEAELRTHIESAMAKIHRYFKETVGGQESDEVAEQWTAQLWSLVDDEDAEALLKEHGV
EESELWPALKSWRGTVAKRTIGPRGRETLDKLMPWLLQEFIQLPTPTKAFLPVSKVLDQI
LTRTTYLELLFENPGARQQLVSLCMASPWIGEQLAKFPMLLDELIDPAQLYDTTSLDDYP
SELRQYLLRVPEEDMEQQMEALRQFKLSQQLKIAAADVTGVLPVMQVSDHLTFLAEAIIE
QVVLQAWQQVSKRHGTPSYLAPEEMGFAVIGYGKAGGLELGYGSDLDLVFLHNYTRDKYP
DAQQTNGDRPIDIGHFYLKLAQRILHLFSTRTTSGELYEVDMRLRPSGASGLLVSEIEYF
GSYQREEAWTWEHQALVRARFLFGDNRLAARFSELRADVLAMERDKAELAKEVREMRQKM
RTHLLKVDEGCFDLKQSPGGIADIEFIAQYLVLANTHDHPELAIWSDNVRIFEVLSELEL
LPHLSAQHLTQAYCYLRDESHRLTLQQAPGQLPQESVDLHVQRVLAIYEQVLNNGQ