Protein Info for Shew_0571 in Shewanella loihica PV-4
Annotation: cytochrome b/b6 domain-containing protein (RefSeq)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 63% identical to CYB_ALLVD: Cytochrome b (petB) from Allochromatium vinosum (strain ATCC 17899 / DSM 180 / NBRC 103801 / NCIMB 10441 / D)
KEGG orthology group: K00412, ubiquinol-cytochrome c reductase cytochrome b subunit [EC: 1.10.2.2] (inferred from 100% identity to slo:Shew_0571)MetaCyc: 48% identical to cytochrome b (Acidithiobacillus ferrooxidans)
RXN-15829 [EC: 7.1.1.8]
Predicted SEED Role
"Ubiquinol--cytochrome c reductase, cytochrome B subunit (EC 1.10.2.2)" in subsystem Ubiquinone Menaquinone-cytochrome c reductase complexes (EC 1.10.2.2)
MetaCyc Pathways
- sn-glycerol 3-phosphate anaerobic respiration (3/3 steps found)
- aerobic respiration I (cytochrome c) (3/4 steps found)
- aerobic respiration II (cytochrome c) (yeast) (3/4 steps found)
- formate to nitrite electron transfer (1/3 steps found)
- Fe(II) oxidation (3/6 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.10.2.2
Use Curated BLAST to search for 1.10.2.2 or 7.1.1.8
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A3QAE5 at UniProt or InterPro
Protein Sequence (404 amino acids)
>Shew_0571 cytochrome b/b6 domain-containing protein (RefSeq) (Shewanella loihica PV-4) MVKNIIDWIDARIPMTATYNRHVGQYATPTNFNFWYFFGSLAMLVLVNQLLTGIWLTMNY VPTAEGAFASIEYIMRDVEYGWLLRYMHSTGASAFFVVVYLHMFRGLIYGSYQKPRELLW LFGMLIFLVLMAEAFMGYLLPWGQMSYWGAQVIISLFGAIPVIGDDLTLWIRGDFVVSGA TLNRFFALHVIALPLVLVVLVFLHLIALHEVGSNNPDGIEIKKNKDENGWPKDGIPFHPY YTVKDIMGVAGFLIVFCYVLFFMPEGGGYFLEKPNFEAANPMKTPEHIAPVWYFTPFYAI LRAIPDKLLGVVGMGLAIAVLFVLPWLDRCKVKSIRYRSMIHKLNIGQFAVSFVILGYLG AVPATPTLTIAARVFTLTYFGFFLALWLYSKNEKTKPVPERLTH