Protein Info for Shew_0567 in Shewanella loihica PV-4

Annotation: GTP-binding protein, HSR1-related (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03156: GTP-binding protein HflX
  • amino acids 11 to 359 (349 residues), 505.3 bits, E=3.8e-156
• PF13167: GTP-bdg_N
  • amino acids 25 to 112 (88 residues), 110.1 bits, E=1.7e-35
• PF16360: GTP-bdg_M
  • amino acids 114 to 192 (79 residues), 103.9 bits, E=1.3e-33
• PF00025: Arf
  • amino acids 198 to 324 (127 residues), 26.2 bits, E=1.3e-09
• PF02421: FeoB_N
  • amino acids 199 to 331 (133 residues), 32.8 bits, E=1.2e-11
• PF01926: MMR_HSR1
  • amino acids 200 to 317 (118 residues), 82 bits, E=8.9e-27

Best Hits

Swiss-Prot: 62% identical to HFLX_ECOLI: GTPase HflX (hflX) from Escherichia coli (strain K12)

KEGG orthology group: K03665, GTP-binding protein HflX (inferred from 100% identity to slo:Shew_0567)

MetaCyc: 62% identical to ribosome rescue factor HflX (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]

Predicted SEED Role

"GTP-binding protein HflX" in subsystem Hfl operon or Universal GTPases

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (14/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (7/8 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.15

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6

Sequence Analysis Tools

See A3QAE1 at UniProt or InterPro

Protein Sequence (432 amino acids)

>Shew_0567 GTP-binding protein, HSR1-related (RefSeq) (Shewanella loihica PV-4)
MFERYEAGEAAVLVHIDFSDEDSREDITELRLLVESAGAETVGVITGSRRSPDRKYFVGT
GKAEELAAMVAATDANVVIFNHALSPAQERNLELICQCRVLDRTTLILDIFAQRARTYEG
KLQVELAQLRHMSTRLIRGWTHLERQKGGIGLRGPGETQLETDRRLLRGRIKTINKRLEK
VDKQREQSRRARQRSELATVSLVGYTNAGKSTLFNALTVSEVYAADQLFATLDPTLRKLD
LQDGSVILADTVGFIRHLPHDLVAAFKATLQETREADLLLHVVDSADENMGDNFEQVQLV
LKEIGAEEIPQLIVCNKIDLLEDVGPKIDYDGDGVPIRVWVSAQQSLGLDLLEKAINQLV
GRAILELTLRIPATAGHYLGQFYRLDVIQQKEYDDLGNCILSVRLLEADWHRLAKQSQGE
LETFIVETAAIE