Protein Info for Shew_0444 in Shewanella loihica PV-4

Annotation: MscS mechanosensitive ion channel (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 540 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details transmembrane" amino acids 283 to 301 (19 residues), see Phobius details amino acids 322 to 345 (24 residues), see Phobius details amino acids 353 to 373 (21 residues), see Phobius details PF00924: MS_channel_2nd" amino acids 371 to 437 (67 residues), 67.8 bits, see alignment E=7.3e-23 PF21082: MS_channel_3rd" amino acids 444 to 526 (83 residues), 62.7 bits, see alignment E=3.6e-21

Best Hits

KEGG orthology group: K03442, small conductance mechanosensitive channel (inferred from 100% identity to slo:Shew_0444)

Predicted SEED Role

"Small-conductance mechanosensitive channel"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3QA18 at UniProt or InterPro

Protein Sequence (540 amino acids)

>Shew_0444 MscS mechanosensitive ion channel (RefSeq) (Shewanella loihica PV-4)
MLRNLALALVTMMALAHLPATADEALQTPQPLTELTQLQQTIDSDIAKLGNANGEMKQFI
EYRIKEHANQLHDKFKLMMNQQPLDKANLLPLLQKHLAFIDKVADYYAADIDQFKQQLGS
DNDTGVMYKLAMRDRQRDEILSAKYETLTWLDALGEDTQAIKTQLTAQLLKRSDMLNSLV
HFTQDKLKLAVDEAAKAGKDVTAEQTALVTNLNERLSLTSASLSVAIELLDAMSQDTTAL
KQTLFSISGDITQDVLNVDVASNLLDQWWNQAKNQALDNGPGLLFKLFVFLLILFLAHLA
GKAVQRIVKKMVSNSKLKFSKLLQDFFVSLSGKTVFALGLMIALSQLGFELGPLLAGFGV
AGVIIGFALQDTLSNFASGMMILIYRPYDVGDLINAAGVTGKVSQMSLVSTTIKTLDNQR
LIIPNNKIWGDTINNITVEHQRRVDMTFGIGYGDNIEKAEQVLRGIVDEHPKVLKQPEPT
VKLHLLGESSVDFVVRPWVKPEDYWDVYWDITRAVKMRFDQEDISIPFPQRDVHIYQTGK