Protein Info for Shew_0443 in Shewanella loihica PV-4

Annotation: formate--tetrahydrofolate ligase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01268: FTHFS
  • amino acids 3 to 563 (561 residues), 784.8 bits, E=1.9e-240

Best Hits

Swiss-Prot: 100% identical to FTHS_SHELP: Formate--tetrahydrofolate ligase (fhs) from Shewanella loihica (strain ATCC BAA-1088 / PV-4)

KEGG orthology group: K01938, formate--tetrahydrofolate ligase [EC: 6.3.4.3] (inferred from 100% identity to slo:Shew_0443)

Predicted SEED Role

"Formate--tetrahydrofolate ligase (EC 6.3.4.3)" in subsystem Folate Biosynthesis or One-carbon metabolism by tetrahydropterines or Serine-glyoxylate cycle (EC 6.3.4.3)

MetaCyc Pathways

• folate transformations II (plants) (11/11 steps found)
• folate transformations III (E. coli) (9/9 steps found)
• folate polyglutamylation (5/5 steps found)
• superpathway of tetrahydrofolate biosynthesis and salvage (10/12 steps found)
• formaldehyde oxidation VII (THF pathway) (4/4 steps found)
• formate assimilation into 5,10-methylenetetrahydrofolate (3/3 steps found)
• folate transformations I (10/13 steps found)
• tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate (2/2 steps found)
• L-histidine degradation III (4/6 steps found)
• reductive glycine pathway of autotrophic CO₂ fixation (6/9 steps found)
• reductive acetyl coenzyme A pathway I (homoacetogenic bacteria) (4/10 steps found)
• purine nucleobases degradation I (anaerobic) (5/15 steps found)
• purine nucleobases degradation II (anaerobic) (11/24 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (21/56 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• One carbon pool by folate

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.4.3

Sequence Analysis Tools

See A3QA17 at UniProt or InterPro

Protein Sequence (564 amino acids)

>Shew_0443 formate--tetrahydrofolate ligase (RefSeq) (Shewanella loihica PV-4)
MLTDIEISRQASLQPIESIATAFGILPHELSPYGRTKAKVDLAITNRIKDNKHGKLVIVT
AVTPTPFGEGKTVTSIGLTQGLQAIGKKSCACIRQPSMGPVFGIKGGAAGGGLAQVVPME
DLNLHLTGDIHAVTSAHNLAAAAIDARLYHEQRLGCEAFREQSGLAPLDIDPEKILWRRV
VDQNERSLRTITVGLGEVNGPVHQGGFDISAASELMAILALSRDLKDLRARIGRIVLAQN
KQGDYISAEDLGVAGAMTAIMSQAISPTLMQTLSGAPCLIHAGPFANIAHGNSSIIADDI
ALRLADIVVTEGGFGSDMGFEKFCNIKTRQSGNAPDAAVLVVTVKALKSHNPDAGSEMDK
LAAGYANLSWHISNVAKYGLPLVVAINRFAEDTQEELEWLKARVLQEGVFACEVCEAFTQ
GAEGAMALARAVVRATEQPSQFRYLYETKQSIEAKLLTIAEAGYGANGISLSDTAKAQLA
QLQRQGFDKLALCIAKTPMSVSHDPKLKGAPQGFELPIAELKLNAGAGFITALVGKVMTM
PGLGIRPGYLNIDIDERGEITGLA