Protein Info for Shew_0389 in Shewanella loihica PV-4

Annotation: pilus (MSHA type) biogenesis protein MshL (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 563 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details PF07655: Secretin_N_2" amino acids 154 to 260 (107 residues), 94.7 bits, see alignment E=3.9e-31 TIGR02519: pilus (MSHA type) biogenesis protein MshL" amino acids 255 to 537 (283 residues), 285.6 bits, see alignment E=1.7e-89 PF00263: Secretin" amino acids 362 to 537 (176 residues), 129.1 bits, see alignment E=1.4e-41

Best Hits

KEGG orthology group: K12282, MSHA biogenesis protein MshL (inferred from 100% identity to slo:Shew_0389)

Predicted SEED Role

"MSHA biogenesis protein MshL" in subsystem Mannose-sensitive hemagglutinin type 4 pilus

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3Q9W3 at UniProt or InterPro

Protein Sequence (563 amino acids)

>Shew_0389 pilus (MSHA type) biogenesis protein MshL (RefSeq) (Shewanella loihica PV-4)
MTAMKTITPLLVLMLAACQTTDRPQPVESKSAIDSSIAAAQNKPQMPPPAAVMPDSVQRE
LQSSALLGSLTPSQPSERRFDVSAKDVDAKVFFPSLVQGTPLSVAVHPNVTGTVSLSLKG
VTLSEALQVVEDIYGYEVSREGRILRVFPAGMRTETFPLNYLYMERQGLSLTSVNSGRIS
DNNNNRNNNGNNGNNSNNYNNNNSNNNGGDSNSNNSNETTNGTFIRSKTKTDFWGELKET
LESIIGNTGDGRQVVVSPQAGLVTIRAYPNELRQVKTFLKTAESHLQRQVILEAKVLEVT
LSDGYQQGIHWESVLGQSGNNSIEFGTSPAPDGLGDVITSAIGGITSLQIKGKDFSTMIN
LLDTQGDVDVLSSPRVTASNNQKAVIKVGRDEYFVTDVSSTTVAGTTPVTTPEVELTPFF
SGIALDVTPQIDEEGSVLLHIHPSVIDIKEQNKSIKVSDSTLELPLAQSEIRESDTVIKA
NTGDVVVIGGLMKSENIELISKVPLLGDIPFLGEAFTNRSNSLRKTELVILLKPTVVGAE
TWQTELQRSKALLDRWYPEEKQE