Protein Info for Shew_0063 in Shewanella loihica PV-4

Annotation: molybdopterin oxidoreductase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 951 signal peptide" amino acids 1 to 53 (53 residues), see Phobius details TIGR01409: Tat (twin-arginine translocation) pathway signal sequence" amino acids 20 to 45 (26 residues), 18.3 bits, see alignment (E = 1.2e-07) PF04879: Molybdop_Fe4S4" amino acids 64 to 112 (49 residues), 41.6 bits, see alignment 1.5e-14 PF00384: Molybdopterin" amino acids 118 to 568 (451 residues), 178.5 bits, see alignment E=3.2e-56 PF01568: Molydop_binding" amino acids 819 to 942 (124 residues), 71.8 bits, see alignment E=6.9e-24

Best Hits

KEGG orthology group: K00123, formate dehydrogenase, alpha subunit [EC: 1.2.1.2] (inferred from 74% identity to fbl:Fbal_3582)

Predicted SEED Role

"Formate dehydrogenase-O, major subunit (EC 1.2.1.2)" in subsystem Formate hydrogenase (EC 1.2.1.2)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.2

Use Curated BLAST to search for 1.2.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A3Q8Y8 at UniProt or InterPro

Protein Sequence (951 amino acids)

>Shew_0063 molybdopterin oxidoreductase (RefSeq) (Shewanella loihica PV-4)
MKLTRKTNEAVATAKPSLGISRRQFMKHAGITTGGIAAASLLGTGMMRRAEAKDIPHDAP
IEIKRTVCSACAVGCGLYAEVQNGVWTGQEPAFDHPFNAGGHCAKGAALREHGHGEKRLK
YPMKLVDGKWKKLSWEQAFNEVGDKMQSIRQESGPDSLYFMGSAKFSNEGCYMYRKFAAM
WGTNNVDHSARICHSTTVAGVANTWGYGAQTNSFNDIQNANAIFFIGSNPAEAHPVAMQH
ILIAKEKNNAKIIVVDPRFSRTAAHSDLHCALRPGTDIPFIYGMLWHIFENGWEDKAFIQ
QRVFEMDTIREEVKKFPPQEVANITGVSEEQVYQAAKLMAENRPGTVVWCMGGTQHHVGN
ANTRAYCILQLALGNMGVSGGGTNIFRGHDNVQGATDLGLLFDTLPGYYGLKTGSWKHWS
HVWDLDFDWVKGRFDQNEYLGRIPMNTPGIPCSRWHDGVLETPEKLAQKDRVRMAFFWGQ
SVNTETRQREVRDALDKMDTVVVVDPFPTMAGVMHRRKDGVYLLPAATQFETEGSVSNSG
RSQQWREKVIEPLFESKTDIEIMYRLSQKLGFDKEYTKRIAKDAKDMLVIEDITREINRG
MWTIGMSGQSPERLKLHTQNWGTFSNKTLEAEGGPAKGETYGLPWPCWGTPEQKHPGTQI
LYNTNKHVLQGGGNFRARYGVEYQGKNLLAEGSFSKGSEIQDGYPEFSDKLLKQLGWWDD
LTAEEKAEAEGKNWKTDLSGGIQRVAMKHGCIPFGNAKARCIVWTFPDQVPVHREPLYTP
RRDLVSKYPTYDDMQVHRLPTLYKSIQEKDTATKYPLGLTSGRLVEYEGGGEESRSNPWL
AELQQEMFVEIHPGDAADRGIRNGDTVWLEGAEGGRIKIKAMVTPRVKQGVTWMPYHFAG
VMHGESLENNYPEGTVPYVIGESANTALTYGYDPVTQMQETKASLCQITKA