Protein Info for Dshi_3895 in Dinoroseobacter shibae DFL-12

Annotation: Gluconate 2-dehydrogenase (acceptor) (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 594 PF01494: FAD_binding_3" amino acids 8 to 43 (36 residues), 27.5 bits, see alignment 7.8e-10 PF00890: FAD_binding_2" amino acids 10 to 47 (38 residues), 32 bits, see alignment 3.2e-11 PF01266: DAO" amino acids 10 to 50 (41 residues), 36.8 bits, see alignment 1.4e-12 PF13450: NAD_binding_8" amino acids 13 to 42 (30 residues), 28.5 bits, see alignment (E = 5.9e-10) PF05199: GMC_oxred_C" amino acids 446 to 574 (129 residues), 60.1 bits, see alignment E=1.4e-19

Best Hits

Swiss-Prot: 47% identical to GADH1_PANCY: Gluconate 2-dehydrogenase flavoprotein from Pantoea cypripedii

KEGG orthology group: K06151, gluconate 2-dehydrogenase alpha chain [EC: 1.1.99.3] (inferred from 100% identity to dsh:Dshi_3895)

MetaCyc: 47% identical to gluconate 2-dehydrogenase dehydrogenase subunit (Pantoea cypripedii)
Gluconate 2-dehydrogenase (acceptor). [EC: 1.1.99.3]

Predicted SEED Role

"Gluconate 2-dehydrogenase (EC 1.1.99.3), membrane-bound, flavoprotein" in subsystem D-gluconate and ketogluconates metabolism (EC 1.1.99.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.99.3

Use Curated BLAST to search for 1.1.99.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LTQ6 at UniProt or InterPro

Protein Sequence (594 amino acids)

>Dshi_3895 Gluconate 2-dehydrogenase (acceptor) (RefSeq) (Dinoroseobacter shibae DFL-12)
MAERRLPKTDVVIVGGGWTGLTAAYELAHAGQRCVVLERGHHRHDATSFGSPEEHDELKY
ALRYGHMQDVKKETLTFRNSRDQRALPMRRLGSFLPGTGLGGAGIHWNGQIWRPLPSDLE
MRSHYEDRYGSQFIPDELSIQDWGVTYDELEPHLDFFEKICGAAGAAGNLRGERQVGGNP
FEGPRSDGYPNPAMKQPISNTKFGRAAERMGLNPFPMPSANVTQAYENPYGAQLHPCTYC
GFCERFGCGYFAKADPIICVYDRIKDHENFEIRFDAQVLRVTKSQDGQTATGVIYLDEDG
VEVFQPADTVCLNAYSLWNVHLMLVSGLGTPYDPETRQGTVGRNYSYQTIAAVDAFFDET
VQTDPFMGAGALGIVVDDFNGDNFDHSDLGFVGGGYIACKQYHGRPISYQPVPEGTPPWG
AEWKAAVRENYTRHADLVIHGSSVSTPENYLDLDPTWTDAYGRPLLRMTFNFPDTDRRMS
DYVMNRAVEIAQEMDNVTSVSSVNLAAEGKNYSIVPYQTTHNVGGAVIGTDPETSVVNRY
GQMWDHHNVFVFGACLFPQNLGYNPTGPLMGLAYWTLEHMKRDYLPNPRPLMDA