Protein Info for Dshi_3832 in Dinoroseobacter shibae DFL-12

Annotation: glucosylglycerol-phosphate synthase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 496 PF00982: Glyco_transf_20" amino acids 4 to 487 (484 residues), 445 bits, see alignment E=1.7e-137 TIGR02398: glucosylglycerol-phosphate synthase" amino acids 8 to 488 (481 residues), 657.5 bits, see alignment E=5.6e-202

Best Hits

Swiss-Prot: 49% identical to GGPS_SYNY3: Glucosylglycerol-phosphate synthase (ggpS) from Synechocystis sp. (strain PCC 6803 / Kazusa)

KEGG orthology group: K03692, glucosylglycerol-phosphate synthase [EC: 2.4.1.213] (inferred from 100% identity to dsh:Dshi_3832)

MetaCyc: 49% identical to glucosylglycerol-phosphate synthase (Synechocystis sp. PCC 6803)
Glucosylglycerol-phosphate synthase. [EC: 2.4.1.213]

Predicted SEED Role

"Alpha,alpha-trehalose-phosphate synthase [UDP-forming] (EC 2.4.1.15)" in subsystem Trehalose Biosynthesis (EC 2.4.1.15)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.15

Use Curated BLAST to search for 2.4.1.15 or 2.4.1.213

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LTJ5 at UniProt or InterPro

Protein Sequence (496 amino acids)

>Dshi_3832 glucosylglycerol-phosphate synthase (RefSeq) (Dinoroseobacter shibae DFL-12)
MSSDLVIVYHRQPYEEVEEDGQTVFRENKSPNGIVPTLKSFFGRVSHGSWIAWKQTETPE
TPEFDRKVEISDDYGNYTVSRLPLAPDQVREFYHVTSKEAFWPILHGFKERFNYDPVEWE
NFRDVNRRFAEAASEEAAEGAVVWIHDYNLWLVPGFLRAMRPDVKIAFFHHTPFPSADIF
NMLPWRKEIIGSLLACDEVGFHIPRYAANFVSVARSLFDVDTPKRHAVPEAWISEGTALT
ERVLPSTITHDGRDVTVTVTPLGVDAAYIDAQARAPATQARAAEIRAEMGETRLLLSVGR
TDYTKGGVQQLESFERVLEARADLRGKVQLMHVSVSANRNMAAYEEIQNEIEQVAGRING
RFGSLDWHPLVLVSRAIPFTDLVAYYRAADVAWITPLADGMNLVCKEFIASRIDGDGVLV
LSEFAGAAVELEDAIITNPFSHKSMDLRIQEALDMDSAERKARMAGLRASVLGNTVQDWK
PQILAALDKPVFDSAA