Protein Info for Dshi_3559 in Dinoroseobacter shibae DFL-12

Annotation: DNA polymerase III, epsilon subunit (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 485 transmembrane" amino acids 12 to 34 (23 residues), see Phobius details amino acids 41 to 68 (28 residues), see Phobius details TIGR00573: exonuclease, DNA polymerase III, epsilon subunit family" amino acids 290 to 455 (166 residues), 98.4 bits, see alignment E=2.2e-32 PF00929: RNase_T" amino acids 292 to 451 (160 residues), 122.8 bits, see alignment E=2.1e-39

Best Hits

KEGG orthology group: K02342, DNA polymerase III subunit epsilon [EC: 2.7.7.7] (inferred from 100% identity to dsh:Dshi_3559)

Predicted SEED Role

"DNA polymerase III epsilon subunit (EC 2.7.7.7)" in subsystem DNA-replication (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LQ52 at UniProt or InterPro

Protein Sequence (485 amino acids)

>Dshi_3559 DNA polymerase III, epsilon subunit (RefSeq) (Dinoroseobacter shibae DFL-12)
MLGTLSLRLRVFLFFAFLAAGGILAVSGALVLGYRQLGDAAALSAFVTSGAVAGFAILAL
VTWVWMLFDENVAKALESLATDMRARAHADIERDLDQNKAKYLGDLAPAAAAVTANLSDM
KNSLAQAVMRETTLLAAEKNHLEAVLNDLPTGVVLCNAQHQVVFYNGRARDLLRPLGGIG
LDRCLASVLDAAPVEAAYAQLLAQPRESCADMRLEVNEGKLRLGVGMRLVEPLPDSALQP
GYILTLREAPMVDTACATPILMPRETAFDFDLLHARPSQDVMDCKLGDLKYVIFDTETTG
LLPERGDEVCQIAACRVVNGKMVKTEMFDTLVNPGRPIPLSATNIHGITDAMVADAPDFL
TAGRAFHGFADGAVLIAHNAPFDMAFFYRKQAEIGARFDHPVLDTVLLSAVIYGQTEQHS
LDALAERLNVTIPEEARHTALGDALATTEVFLKLLPMLQAKGIETFGDLLAQTKKHARLL
EDLNA