Protein Info for Dshi_3557 in Dinoroseobacter shibae DFL-12

Annotation: putative CBS domain and cyclic nucleotide-regulated nucleotidyltransferase (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 605 PF00027: cNMP_binding" amino acids 38 to 116 (79 residues), 51.4 bits, see alignment E=1.8e-17 PF00571: CBS" amino acids 143 to 197 (55 residues), 47.4 bits, see alignment 4e-16 amino acids 206 to 258 (53 residues), 50.2 bits, see alignment 5.3e-17 PF03445: DUF294" amino acids 285 to 420 (136 residues), 157.1 bits, see alignment E=5.1e-50 PF10335: DUF294_C" amino acids 458 to 597 (140 residues), 150.5 bits, see alignment E=5.9e-48

Best Hits

KEGG orthology group: K07182, CBS domain-containing protein (inferred from 100% identity to dsh:Dshi_3557)

Predicted SEED Role

"Predicted signal-transduction protein containing cAMP-binding and CBS domains" in subsystem cAMP signaling in bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LQ50 at UniProt or InterPro

Protein Sequence (605 amino acids)

>Dshi_3557 putative CBS domain and cyclic nucleotide-regulated nucleotidyltransferase (RefSeq) (Dinoroseobacter shibae DFL-12)
MQDLTKIQSFLAHVHPYDSLTDAELAILAGQVEVVTQAAGEKIYTLGEPCRGVYVIVSGE
VEITDETGAQISQLGSRNSFGERGLARDGVAVTNARAASDTDMLLVPAEAFHELVDVHPA
IAKFFNRSRGAETRKTDLATARISELMTRQVLGITADATIQRAAHLMHAKKVSSLAVLEG
GRLVGIVTVRDMARVVSEGLANDAPVSEVMARKIISLPPDALGSDVLHTMLERRIGHLPI
TDKGTLVGIVTQTDLIRFQALSSAQLVHDIVYADTPSGIAAVTKRLPELLAQLVGAGNPH
EVVTRLITDIADAATRRLLALAEEKLGPPPVPYLWLACGSQGRQEQTGVSDQDNCLILDD
AMTPEMDGYFKALASFVCDGLNAAGYVYCPGDMMATADRWRQPLRVWKQYFQGWIAQPDT
EAQMLASVMFDLRPIGGEDSLFANLQDETLAAASANSIFVTHMIANSLKHTPPLGMFRGF
ATIRSGEHKNALDLKHNGVVPVVDLGRIYALRGRLTAVNTRARLLAARDEKVISPSGAAD
LLDAYDLIAETRLQHQARQVRAGEAPDNFMSPTTLSDFERSHLRDAFVVIRTMQSAAGSG
RGGLA