Protein Info for Dshi_3535 in Dinoroseobacter shibae DFL-12

Annotation: light-independent protochlorophyllide reductase, B subunit (RefSeq)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 528 TIGR01278: light-independent protochlorophyllide reductase, B subunit" amino acids 1 to 526 (526 residues), 608 bits, see alignment E=8.1e-187 PF00148: Oxidored_nitro" amino acids 12 to 405 (394 residues), 275.5 bits, see alignment E=6.7e-86 PF08369: PCP_red" amino acids 479 to 523 (45 residues), 69.8 bits, see alignment 1.6e-23

Best Hits

Swiss-Prot: 100% identical to BCHB_DINSH: Light-independent protochlorophyllide reductase subunit B (bchB) from Dinoroseobacter shibae (strain DSM 16493 / NCIMB 14021 / DFL 12)

KEGG orthology group: K04039, light-independent protochlorophyllide reductase subunit B [EC: 1.18.-.-] (inferred from 100% identity to dsh:Dshi_3535)

MetaCyc: 69% identical to light-independent protochlorophyllide reductase subunit B (Rhodobacter capsulatus)
RXN-11902 [EC: 1.3.7.7]; 1.3.7.7 [EC: 1.3.7.7]

Predicted SEED Role

"Light-independent protochlorophyllide reductase subunit B (EC 1.18.-.-)" in subsystem Chlorophyll Biosynthesis (EC 1.18.-.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.18.-.-, 1.3.7.7

Use Curated BLAST to search for 1.18.-.- or 1.3.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A8LQ28 at UniProt or InterPro

Protein Sequence (528 amino acids)

>Dshi_3535 light-independent protochlorophyllide reductase, B subunit (RefSeq) (Dinoroseobacter shibae DFL-12)
MKLTVWTYEGPPHVGAMRVATGMTGLHYVLHAPQGDTYADLLFTMIERRDHRPPVSYTTF
QARDLGSDTAHLFKDSCRDAYERFKPEAIIVGASCTAELIQDDPGGLAETMGLPIPVIAL
ELPSYQRKENFGCDETFFQIVRALAKPVEKTARVSCNILGPTGLGFRHRDDVEELTGLLS
EMGVDVNVVAPMRSSPSDIARLGAAHFNVMLYPETCEAACRHLERAFQQPYTKTVPIGVG
ATRDFIAEVQGLTGVTGAPDENRLRLPWWSASVDSTYLTGKRVFLFGDATHVKASARIAR
DEMGFEVVGLGCYNREFARDIRKLAKEFGLEALITDDYLEVEKAIEEAAPEMILGTQMER
HIGKRLGIPCAVISAPVHVQDFPARYSPQVGFEGANVIFDTWIHPLVMGLEEHLLAMFRE
DFEFHDAAGPSHHGGHAPKPMHDAPAASAAAGAEASMAEETAAPSQDAPAATGGDVTVWL
ADAEKELKKIPFFVRGKAKRNTEKYALEQGVTEISVDTLYEAKAHYAR